Department of NMR & MRI Facility, All India Institute of Medical Sciences, New Delhi, India.
NMR Biomed. 2014 Jan;27(1):80-9. doi: 10.1002/nbm.2979. Epub 2013 Jul 4.
High-resolution NMR spectroscopic studies of prostate tissue extracts, prostatic fluid, seminal fluid, serum and urine can be used for the detection of prostate cancer, based on the differences in their metabolic profiles. Useful diagnostic information is obtained by the detection or quantification of as many metabolites as possible and comparison with normal samples. Only a few studies have shown the potential of high-resolution in vitro NMR of prostate tissues. A survey of the literature has revealed that studies on body fluids, such as urine and serum, in relation to prostate cancer are rare. In addition, the potential of NMR of nuclei other than (1)H, such as (13)C and (31)P, has not been exploited fully. The metabolomic analysis of metabolites, detected by high-resolution NMR, may help to identify metabolites which could serve as useful biomarkers for prostate cancer detection. Such NMR-derived biomarkers would not only help in prostate cancer detection and in understanding the in vivo MRS metabolic profile, but also to investigate the biochemical and metabolic changes associated with cancer. Here, we review the published research work on body fluids in relation to prostate and prostate tissue extracts, and highlight the potential of such studies for future work.
基于代谢谱的差异，利用高分辨率 NMR 光谱技术对前列腺组织提取物、前列腺液、精液、血清和尿液进行研究，可用于前列腺癌的检测。通过尽可能多地检测和定量检测代谢物，并与正常样本进行比较，可以获得有用的诊断信息。只有少数研究表明高分辨率体外 NMR 对前列腺组织具有潜在的应用价值。对文献的调查显示，与前列腺癌相关的尿液和血清等体液的研究很少。此外，尚未充分利用 (1)H 以外的核（如 (13)C 和 (31)P）的 NMR 潜力。通过高分辨率 NMR 检测到的代谢物的代谢组学分析，可能有助于鉴定可作为前列腺癌检测有用生物标志物的代谢物。这些源自 NMR 的生物标志物不仅有助于前列腺癌的检测和理解体内 MRS 代谢谱，而且有助于研究与癌症相关的生化和代谢变化。在这里，我们综述了与前列腺和前列腺组织提取物相关的体液的已发表研究工作，并强调了这些研究对未来工作的潜力。
