Department of Medical Chemistry, Medical University of Białystok, Poland.
Adv Clin Exp Med. 2013 May-Jun;22(3):347-53.
Helicobacter pylori is responsible for gastroduodenal diseases such as chronic gastritis, gastric and duodenal ulcers and also gastric malignances. During an infection, gastric mucins, especially secretory MUC5AC, are said to participate in interactions with bacterial adhesins. Recently, epithelial MUC1 mucin has also been proposed to be engaged in infection development. H. pylori surface possesses lipopolisaccharides with Lewis antigens whose role in interactions with gastric mucins has not been elucidated thoroughly so far.
To check the involvement of Lewis b and H type 1 structures of H. pylori in adhesion of bacteria to MUC1 mucin.
The study was performed on gastric juices taken from 10 clinical patients and 4 H. pylori strains. Bacteria were assessed for the presence of Lewis b and H type 1 structures by ELISA test. Interactions of H. pylori with MUC1 were analyzed by sandwich ELISA method with bacteria pretreated or not with Lewis b - HSA or H type 1 - HSA glycoconjugates.
In the majority of the patients, the slight increase of adhesion of H. pylori to MUC1, after pretreatment of bacteria with H type 1 glycoconjugate was observed. A statistically significant difference was revealed in one strain with a dose of conjugate 50µg/mL (P = 0.05). The influence of Lewis b on adhesion is considered to be contradictory.
H type 1 antigens are suggested to be involved in carbohydrate - carbohydrate interactions of H. pylori with MUC1 mucin.
幽门螺杆菌是导致胃十二指肠疾病的元凶，如慢性胃炎、胃溃疡和十二指肠溃疡，也与胃部恶性肿瘤相关。在感染期间，胃粘蛋白，特别是分泌型 MUC5AC，被认为参与了与细菌黏附素的相互作用。最近，上皮型 MUC1 粘蛋白也被认为参与了感染的发展。幽门螺杆菌表面存在带有 Lewis 抗原的脂多糖，其与胃粘蛋白相互作用的作用迄今尚未完全阐明。
检查幽门螺杆菌表面的 Lewis b 和 H 型 1 结构是否参与细菌与 MUC1 粘蛋白的黏附。
本研究使用了从 10 名临床患者和 4 株幽门螺杆菌中提取的胃液。通过 ELISA 试验评估细菌中是否存在 Lewis b 和 H 型 1 结构。通过夹心 ELISA 法分析了未预处理或用 Lewis b-HSA 或 H 型 1-HSA 糖缀合物预处理的细菌与 MUC1 的相互作用。
在大多数患者中，经 H 型 1 糖缀合物预处理后，幽门螺杆菌与 MUC1 的黏附略有增加。在一株细菌的 50μg/ml 浓度下，差异具有统计学意义（P=0.05）。Lewis b 的影响被认为是矛盾的。
提示 H 型 1 抗原参与了幽门螺杆菌与 MUC1 粘蛋白的碳水化合物-碳水化合物相互作用。
