Department of Medical Chemistry, Medical University of Białystok, ul Mickiewicza 2a, 15-230, Białystok 8, Poland,
Mol Cell Biochem. 2014 Jan;385(1-2):249-55. doi: 10.1007/s11010-013-1833-1. Epub 2013 Oct 6.
To assess the influence of monoclonal anti-Lewis b, anti-H type 1, and anti-sialyl Lewis x addition on interactions of sugar structures of MUC1 mucin with Helicobacter pylori. The investigations were carried out on gastric juices of 11 patients and 12 H. pylori strains. The levels of Lewis b and sialyl Lewis x antigens on MUC1 were assessed by sandwich ELISA tests. Anti-Lewis b, anti-H type 1 or anti-sialyl Lewis x monoclonal antibodies were added to MUC1 to determine whether the adhesion activities of H. pylori isolates to examined mucin would be affected. Binding of bacteria to MUC1 was assessed by ELISA test. Clear inhibitory effect of examined antibodies was revealed in 6 of 12 examined H. pylori isolates independently on babA2 status. In the rest of strains this effect was negligible. We confirmed participation of Lewis b, H type 1 and also sialyl Lewis x of MUC1 mucin in interactions with H. pylori independently on babA genopositivity. Not full inhibition and a lack of this effect in some strains suggest an existence of other mechanisms of H. pylori adherence to mucin.
为了评估单克隆抗-Lewis b、抗-H 型 1 和抗唾液酸化 Lewis x 添加物对 MUC1 粘蛋白糖结构与幽门螺杆菌相互作用的影响。研究在 11 名患者和 12 株幽门螺杆菌菌株的胃液中进行。通过夹心 ELISA 试验评估 MUC1 上 Lewis b 和唾液酸化 Lewis x 抗原的水平。将抗-Lewis b、抗-H 型 1 或抗唾液酸化 Lewis x 单克隆抗体添加到 MUC1 中，以确定幽门螺杆菌分离株对所检查粘蛋白的粘附活性是否会受到影响。通过 ELISA 试验评估细菌与 MUC1 的结合。在 12 株被检查的幽门螺杆菌分离株中，有 6 株独立于 babA2 状态显示出明显的抑制作用。在其余的菌株中，这种作用可以忽略不计。我们证实了 Lewis b、H 型 1 以及 MUC1 粘蛋白的唾液酸化 Lewis x 参与了与幽门螺杆菌的相互作用，而 babA 基因阳性与否并不影响这一作用。不完全抑制和在一些菌株中缺乏这种作用表明幽门螺杆菌与粘蛋白结合的其他机制的存在。
