Department of Hepatobiliary Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China.
Pathol Oncol Res. 2014 Jan;20(1):73-9. doi: 10.1007/s12253-013-9660-y. Epub 2013 Jul 5.
To investigate the expressions of casein kinase II β (CK2β) and X-Linked inhibitor of apoptosis protein (XIAP) in cholangiocarcinoma (CCA) and evaluated their correlations with major clinicopathologic features and patients' survival. Fifty CCA specimens and 20 normal liver tissues were included in the study. Immunohistochemical staining was used to determine the expression levels of CK2β, XIAP in normal and CCA tissues. The relationships of CK2β and XIAP expressions with clinicopathologic parameters and clinical outcome were evaluated. High immunostaining of CK2β and XIAP were observed in 66% (33/50) and 68% (34/50) of CCA tissues, which were significantly higher than that of normal liver tissues 0% (0/20) and 25% (5/20). The high expression of CK2β was significantly associated with TNM stage (P = 0.036), histological grade (P = 0.020) and high serum CEA level (P = 0.010), while high expression of XIAP was only associated with TNM stage (P = 0.014) and high serum CEA level (P = 0.001). By univariant analysis, patients with high expression of CK2β and XIAP demonstrate significantly poorer overall survival (P = 0.003 vs P = 0.018). Cox regression model showed that positive expression of CK2βis an independent factor of prognosis (P = 0.004). The expressions of CK2β and XIAP in CCA tissues showed strong correlations with the tumor progression, CK2β may be applied as a potential prognostic marker for CCA.
研究酪蛋白激酶 IIβ（CK2β）和 X 连锁凋亡抑制蛋白（XIAP）在胆管癌（CCA）中的表达情况，并评估其与主要临床病理特征和患者生存的关系。方法：收集 50 例 CCA 标本和 20 例正常肝组织，采用免疫组织化学染色法检测 CK2β、XIAP 在正常和 CCA 组织中的表达水平，评估 CK2β、XIAP 表达与临床病理参数和临床结局的关系。结果：CCA 组织中 CK2β和 XIAP 的高免疫染色率分别为 66%（33/50）和 68%（34/50），明显高于正常肝组织的 0%（0/20）和 25%（5/20）。CK2β 的高表达与 TNM 分期（P=0.036）、组织学分级（P=0.020）和高血清 CEA 水平（P=0.010）显著相关，而 XIAP 的高表达仅与 TNM 分期（P=0.014）和高血清 CEA 水平（P=0.001）相关。单因素分析显示，CK2β 和 XIAP 高表达患者的总生存率明显较低（P=0.003 与 P=0.018）。Cox 回归模型显示，CK2β 阳性表达是预后的独立因素（P=0.004）。CCA 组织中 CK2β 和 XIAP 的表达与肿瘤进展密切相关，CK2β 可能成为 CCA 的潜在预后标志物。
