Institute of Microecology, Poznań, Poland.
Pol J Microbiol. 2013;62(1):91-5.
The aim of the study was evaluation of the correlation between the level of clinical activity of Crohn's disease (CD) and the number of Faecalibacterium prausnitzii, total number of bacteria and the concentration of selected short chain fatty acids (SCFA) in stool. 34 individuals diagnosed with Crohn's disease participated in this study in 2011. The disease activity was determined according to the Crohn Disease Activity Index (CDAI). The number of Faecalibacterium prausnitzii and total number of bacteria were monitored by RT-PCR. The concentrations of SCFA were determined by gas chromatography. In CD patients, Faecalibacterium prausnitzii number and percentage of the total number of bacteria were greatly reduced. In patients with CD the percentage of acetate was elevated (70%), while the percentages of propionate and butyrate were significantly reduced (14.9% and 7.99%, respectively).
本研究的目的是评估克罗恩病（CD）的临床活动水平与粪便中产双歧杆菌数量、总细菌数量和选定短链脂肪酸（SCFA）浓度之间的相关性。2011 年，34 名确诊为 CD 的患者参与了本研究。疾病活动度根据克罗恩病活动指数（CDAI）进行评估。通过 RT-PCR 监测产双歧杆菌数量和总细菌数量。通过气相色谱法测定 SCFA 浓度。在 CD 患者中，产双歧杆菌数量和总细菌数量的百分比大大减少。在 CD 患者中，乙酸盐的百分比升高（70%），而丙酸盐和丁酸盐的百分比显著降低（分别为 14.9%和 7.99%）。
