Cao Yuan, Shen Jun, Ran Zhi Hua
Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institution of Digestive Disease, Shanghai Inflammatory Bowel Disease Research Center, Shanghai 200127, China.
Gastroenterol Res Pract. 2014;2014:872725. doi: 10.1155/2014/872725. Epub 2014 Mar 27.
Background. Laboratory data suggests a reduction of Faecalibacterium prausnitzii (F. prausnitzii) is confirmed both in fecal samples in inflammatory bowel disease (IBD) patients. Numerous observational studies have suspected dysbiosis, an imbalance between protective and harmful bacteria to be relevant to the etiology and pathogenesis of IBD. Methods. Medline, EMBASE, Pubmed, and others. were searched by 2 independent reviewers. Of 48 abstracts reviewed, 11 studies met our inclusion criteria (subject N = 1180). Meta-analysis was performed with Review Manager 5.2. Results. The bacterial count of F. prausnitzii in IBD patients was significantly lower (6.7888 ± 1.8875) log10 CFU/g feces than healthy controls (7.5791 ± 1.5812) log10 CFU/g feces; P < 0.0001. The Standardization Mean Difference of F. prausnitzii in IBD patients was -0.94 (95% confidence interval [CI]: -1.07--0.80). Subgroup analyses revealed a trend toward a greater effect for CD (SMD: -1.13, 95% CI: -1.32--0.94) when compared to UC (SMD: -0.78, 95% CI: -0.97--0.60). Conclusions. The abundance of F. prausnitzii was decreased in IBD patients compared with healthy controls. Furthermore, the reduction of F. prausnitzii and misbalance of the intestinal microbiota are particularly higher in CD patients with ileal involvement.
背景。实验室数据表明，炎症性肠病（IBD）患者粪便样本中普拉梭菌（F. prausnitzii）数量减少已得到证实。众多观察性研究怀疑，保护性细菌与有害细菌之间的失衡——即生态失调——与IBD的病因和发病机制相关。方法。由两名独立审阅者检索医学期刊数据库（Medline）、荷兰医学文摘数据库（EMBASE）、美国国立医学图书馆生物医学信息数据库（Pubmed）等。在审阅的48篇摘要中，11项研究符合我们的纳入标准（受试者N = 1180）。使用RevMan 5.2进行荟萃分析。结果。IBD患者粪便中普拉梭菌的细菌计数（6.7888 ± 1.8875）log10 CFU/g粪便显著低于健康对照（7.5791 ± 1.5812）log10 CFU/g粪便；P < 0.0001。IBD患者中普拉梭菌的标准化均数差为 -0.94（95%置信区间[CI]：-1.07--0.80）。亚组分析显示，与溃疡性结肠炎（UC）（标准化均数差：-0.78，95% CI：-0.97--0.60）相比，克罗恩病（CD）有更大效应的趋势（标准化均数差：-1.13，95% CI：-1.32--0.94）。结论。与健康对照相比，IBD患者中普拉梭菌丰度降低。此外，在累及回肠的CD患者中，普拉梭菌减少和肠道微生物群失衡尤其更严重。
