Congur Gulsah, Senay Hilal, Turkcan Ceren, Canavar Ece, Erdem Arzum, Akgol Sinan
Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova, 35100 Izmir, Turkey.
Comb Chem High Throughput Screen. 2013 Jun 28;16(7):503-10. doi: 10.2174/1386207311316070001.
The aim of this study is (i) to prepare estrone-imprinted nanospheres (nano-EST-MIPs) and (ii) to integrate them into the electrochemical sensor as a recognition layer. N-methacryloyl-(l)-phenylalanine (MAPA) was chosen as the complexing monomer. Firstly, estrone (EST) was complexed with MAPA and the EST-imprinted poly(2-hyroxyethylmethacrylate-co-N-methacryloyl-(l)-phenylalanine) [EST-imprinted poly(HEMA-MAPA)] nanospheres were synthesized by surfactant- free emulsion polymerization method. The specific surface area of the EST-imprinted poly(HEMA-MAPA) nanospheres was found to be 1275 m2/g with a size of 163.2 nm in diameter. According to the elemental analysis results, the nanospheres contained 95.3 mmole MAPA/g nanosphere. The application of EST specific MIP nanospheres for the development of an electrochemical biosensor was introduced for the first time in our study by using electrochemical impedance spectroscopy (EIS) technique. This nano-MIP based sensor presented a great specificity and selectivity for EST.
（i）制备雌酮印迹纳米球（纳米-EST-MIPs）；（ii）将其作为识别层集成到电化学传感器中。选择N-甲基丙烯酰基-（l）-苯丙氨酸（MAPA）作为络合单体。首先，使雌酮（EST）与MAPA络合，并通过无表面活性剂乳液聚合法合成了EST印迹聚（甲基丙烯酸2-羟乙酯-co-N-甲基丙烯酰基-（l）-苯丙氨酸）[EST印迹聚（HEMA-MAPA）]纳米球。发现EST印迹聚（HEMA-MAPA）纳米球的比表面积为1275 m2/g，直径为163.2 nm。根据元素分析结果，纳米球每克含有95.3毫摩尔MAPA。在我们的研究中，首次通过电化学阻抗谱（EIS）技术介绍了EST特异性MIP纳米球在电化学生物传感器开发中的应用。这种基于纳米MIP的传感器对EST具有很高的特异性和选择性。
