Sobral B W, Sadowsky M J, Atherly A G
Department of Genetics, Iowa State University, Ames 50011.
Appl Environ Microbiol. 1990 Jun;56(6):1949-53. doi: 10.1128/aem.56.6.1949-1953.1990.
The genomes of 11 Bradyrhizobium japonicum serocluster 123 field isolates were analyzed by using field inversion gel electrophoresis. Genomic fingerprints produced by digestion of intact genomic DNA in agarose plugs with the rare-cutting restriction enzymes AseI, DraI, SpeI, and XbaI showed that the isolates were genetically diverse. Few (30 to 50%) isolates exhibited the same fingerprint as the USDA serogroup strain with which they are antigenically related. Southern hybridization with a nifHD gene probe to the blotted field inversion electrophoresis gels provided further evidence of the relatedness between members of serogroups 123 and 127.
利用脉冲场凝胶电泳对11株日本慢生根瘤菌血清群123田间分离株的基因组进行了分析。用稀有切割限制酶AseI、DraI、SpeI和XbaI消化琼脂糖凝胶块中的完整基因组DNA所产生的基因组指纹图谱表明，这些分离株在遗传上具有多样性。很少（30%至50%）的分离株表现出与其抗原相关的美国农业部血清群菌株相同的指纹图谱。用nifHD基因探针与印迹的脉冲场电泳凝胶进行Southern杂交，进一步证明了血清群123和127成员之间的相关性。
