University of Warsaw, Department of Chemistry, Warsaw, Poland.
Anal Chim Acta. 2013 Jul 17;787:118-25. doi: 10.1016/j.aca.2013.05.052. Epub 2013 Jun 8.
For the needs of photometric determination of creatinine according to Jaffe protocol a dedicated paired emitter detector diode (PEDD) detector has been developed. This PEDD device has been constructed in the compact form of flow-through cell (30 μL total volume and 7 mm optical pathlength) integrated with 505 nm LED-based emitter and 525 nm LED-based detector compatible with multicommutated flow analysis (MCFA) system. This fully mechanized MCFA system configured of microsolenoid valves and pumps is operating under microprocessor control. The developed analytical system offers determination of creatinine in the submillimolar range of concentrations with detection limit at ppm level. The throughput offered by the system operating according to multi-point fixed-time procedure for kinetic measurements is 15-40 samples per hour depending on the mode of measurements. The developed PEDD-based MCFA system has been successfully applied for the determination of creatinine in real samples of human urine as well as serum. The developed sampling unit used the system is free from effects caused by differences in sample viscosity.
为了满足根据 Jaffe 方案进行肌酐光度测定的需求，我们开发了一种专用的配对发射器检测器二极管（PEDD）检测器。该 PEDD 设备采用流通池的紧凑形式构建（总体积为 30μL，光程长度为 7mm），集成了基于 505nm LED 的发射器和 525nm LED 的检测器，与多切换流动分析（MCFA）系统兼容。该完全机械化的 MCFA 系统由微电磁阀和泵组成，在微处理器控制下运行。开发的分析系统可在亚毫摩尔浓度范围内测定肌酐，检测限可达 ppm 级。根据多点固定时间动力学测量程序运行的系统的吞吐量为每小时 15-40 个样品，具体取决于测量模式。所开发的基于 PEDD 的 MCFA 系统已成功应用于人体尿液和血清中肌酐的测定。所开发的采样单元在使用该系统时不受样品粘度差异引起的影响。
