在酶联免疫吸附测定法中使用重组核糖核蛋白肽70K和A检测混合性结缔组织病中的抗体：18例患者的纵向随访
Use of recombinant RNP peptides 70K and A in an ELISA for measurement of antibodies in mixed connective tissue disease: a longitudinal follow up of 18 patients.
作者信息
de Rooij D J, Habets W J, van de Putte L B, Hoet M H, Verbeek A L, van Venrooij W J
机构信息
Department of Internal Medicine, University Hospital, Nijmegen, The Netherlands.
出版信息
Ann Rheum Dis. 1990 Jun;49(6):391-5. doi: 10.1136/ard.49.6.391.
Abstract
In a three year prospective study disease activity variables and levels of antibody against the RNP-peptides 70K and A were measured in 18 patients with mixed connective tissue disease. Antibody measurement entailed use of cloned autoantigens in an enzyme linked immunosorbent assay (ELISA). Fluctuations in antibody levels against 70K and A were most commonly noted in patients who also had changes in disease activity, but these changes in serology and disease activity were synchronous in only a minority of the episodes. Even major disease flares were associated with changes in anti-A levels in only a few, and with changes in anti-70K levels in none of the episodes. The data indicate that measurements of anti-70K and anti-A levels are not useful in monitoring disease activity or response to treatment in mixed connective tissue disease, and suggest that these antibody specificities do not play a direct part in the pathogenesis of disease manifestations.
摘要
在一项为期三年的前瞻性研究中，对18例混合性结缔组织病患者的疾病活动变量以及抗RNP肽70K和A的抗体水平进行了测量。抗体测量采用酶联免疫吸附测定（ELISA）法使用克隆的自身抗原。在疾病活动也有变化的患者中，最常观察到抗70K和抗A抗体水平的波动，但在少数发作中，血清学和疾病活动的这些变化是同步的。即使是严重的疾病发作，也仅有少数与抗A水平变化相关，无一与抗70K水平变化相关。数据表明，在混合性结缔组织病中，测量抗70K和抗A水平对监测疾病活动或治疗反应并无用处，并提示这些抗体特异性在疾病表现的发病机制中不发挥直接作用。
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