Grigor'ev A E, Liutskanova D G, Todorov T Kh, Danilenko V N
Antibiot Khimioter. 1990 Apr;35(4):10-4.
Grossing of S. erythraeus 4 with S. erythraeus 1 resulted in transfer of genetic elements from strain 4 to strain 1 as evidence by the 20 and 18 kb fragments in the experiments on DNA-DNA hybridization. The presence of the genetic elements in strain 1 was the cause of plasmid pSE 21 mobility. In strain 6, a derivative of S. erythraeus 1 plasmid pSE 21 was accompanied by other extrachromosomal DNAs characterized by high instability. During storage of the strain at a temperature of 4 degrees C for more than 1 or 2 months the number of the plasmid pSE 21 copies decreased. When the strain was stored for longer periods (6 months or more) the plasmid DNA was not detectable even with the DNA-DNA hybridization procedure. The results of hybridization of a fraction of the extrachromosomal DNA of S. erythraeus 6, the Bam HIB fragment of plasmid pSE 21 with the total DNA of strains 1, 4, 5, 6 and BTCC 2 of S. erythraeus and hybridization of DNA of plasmid pSE 21 with the total DNA of S. erythraeus 6 and 1 showed that (1) strains 1, 5 and BTCC 2 had the same hybridization patterns, (2) the other extrachromosomal DNAs present in the fraction were homologous with the Bam HIA fragment of plasmid pSE 21, (3) chromosomes of strains 1, 4, 5, 6 and BTCC 2 of S. erythraeus also contained DNA homologous to the plasmid Bam HIA fragment. It was suggested that plasmid pSE 21 could be used as a basis for constructing the integrative vector for S. erythraeus.
将红链霉菌4与红链霉菌1进行菌苔混合培养，结果导致遗传元件从菌株4转移至菌株1，这一结果在DNA - DNA杂交实验中表现为20 kb和18 kb的片段。菌株1中遗传元件的存在是质粒pSE 21具有迁移性的原因。在菌株6中，它是红链霉菌1的衍生物，质粒pSE 21伴随着其他具有高度不稳定性特征的染色体外DNA。当该菌株在4℃温度下保存超过1或2个月时，质粒pSE 21的拷贝数减少。当菌株保存更长时间（6个月或更长时间）时，即使采用DNA - DNA杂交方法也检测不到质粒DNA。红链霉菌6的一部分染色体外DNA（质粒pSE 21的Bam HIB片段）与红链霉菌1、4、5、6和BTCC 2的总DNA杂交，以及质粒pSE 21的DNA与红链霉菌6和1的总DNA杂交的结果表明：（1）菌株1、5和BTCC 2具有相同的杂交模式；（2）该部分中存在的其他染色体外DNA与质粒pSE 21的Bam HIA片段同源；（3）红链霉菌1、4、5、6和BTCC 2的染色体也含有与质粒Bam HIA片段同源的DNA。有人提出质粒pSE 21可作为构建红链霉菌整合载体的基础。
