School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, China.
Steroids. 2013 Oct;78(10):1015-20. doi: 10.1016/j.steroids.2013.06.007. Epub 2013 Jul 4.
Five new polyhydroxypregnane glycosides, namely cynanotosides A-E (1-5), together with two known analogues, deacetylmetaplexigenin (6) and cynotophylloside H (7), were isolated from the roots of Cynanchum otophyllum. Their structures were established by spectroscopic methods and acid hydrolysis. The neuroprotective effects of compounds 1-7 against glutamate-, hydrogen peroxide-, and homocysteic acid (HCA)-induced cell death were tested by MTT assay in a hippocampal neuronal cell line HT22. Compounds 1, 2, and 7 exhibited protective activity against HCA-induced cell death in a dose-dependent manner ranging from 1 to 30μM, which may explain the Traditional Chinese Medicine (TCM) use of this plant for the treatment of epilepsy.
从鹅绒藤根部分离得到五个新的多羟基孕甾烷糖苷，分别命名为 Cynanotosides A-E（1-5），以及两个已知类似物，去乙酰基 metaplexigenin（6）和 cynotophylloside H（7）。通过光谱方法和酸水解确定了它们的结构。通过 MTT 法在海马神经元细胞系 HT22 中测试了化合物 1-7 对谷氨酸、过氧化氢和高半胱氨酸（HCA）诱导的细胞死亡的神经保护作用。化合物 1、2 和 7 对 HCA 诱导的细胞死亡表现出剂量依赖性的保护活性，范围从 1 到 30μM，这可能解释了这种植物在传统中药（TCM）中用于治疗癫痫的用途。
