Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon, 200-701, Korea.
Arch Pharm Res. 2012 Mar;35(4):617-21. doi: 10.1007/s12272-012-0404-4. Epub 2012 May 3.
Ten compounds were isolated from the 80% methanol extract of roots of Cynanchum paniculatum Kitagawa (Asclepiadaceae) and identified as paeonol (1), 4-acetylphenol (2), 2,5-dihydroxy-4-methoxyacetophenone (3), 2,3-dihydroxy-4-methoxyacetophenone (4), acetoveratrone (5), 2,5-dimethoxyhydroquinone (6), vanillic acid (7), resacetophenone (8), m-acetylphenol (9) and 3,5-dimethoxyhydroquinone (10). The protective effect of compounds 1-10 against glutamate-induced cytotoxicity in hippocampal HT22 cell line was evaluated. Among them, compound 4 showed a relatively effective protection of 47.55% (at 10 μM). This result suggested that compounds 4 had neuroprotective effect on the glutamate-induced neurotoxicity in HT22 cells.
从杠柳（夹竹桃科）根的 80%甲醇提取物中分离得到 10 种化合物，分别鉴定为丹皮酚（1）、4-乙酰苯酚（2）、2,5-二羟基-4-甲氧基苯乙酮（3）、2,3-二羟基-4-甲氧基苯乙酮（4）、乙酰香草酮（5）、2,5-二甲氧基对苯二酚（6）、香草酸（7）、反式-乙酰苯醌（8）、m-乙酰苯酚（9）和 3,5-二甲氧基对苯二酚（10）。评价了化合物 1-10 对海马 HT22 细胞系谷氨酸诱导细胞毒性的保护作用。其中，化合物 4 表现出相对有效的保护作用（在 10 μM 时为 47.55%）。该结果表明化合物 4 对 HT22 细胞中谷氨酸诱导的神经毒性具有神经保护作用。
