Kim Jeong-Hwan, Kang Seong-Il, Shin Hye-Sun, Yoon Seon-A, Kang Seung-Woo, Ko Hee-Chul, Kim Se-Jae
Department of Biology, Jeju National University, Jeju 690-756, Republic of Korea.
Biosci Biotechnol Biochem. 2013;77(7):1595-8. doi: 10.1271/bbb.130167. Epub 2013 Jul 7.
In this study, we examined the effects of Jeju dwarf bamboo (Sasa quelpaertensis Nakai) extract (JBE) and p-coumaric acid (CA) on oleic acid (OA)-induced lipid accumulation in HepG2 cells. JBE and CA increased the phosphorylation of AMP-activated protein kinase (AMPK), and acetyl-CoA carboxylase (ACC) and the expression of carnitine palmitoyl transferase 1a (CPT1a) in OA-treated HepG2 cells. Additionally, these compounds decreased sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid synthase (FAS), and OA-induced lipid accumulation, suggesting that JBE and CA modulate lipid metabolism in HepG2 cells via the AMPK activation pathway.
在本研究中，我们检测了济州矮竹（Sasa quelpaertensis Nakai）提取物（JBE）和对香豆酸（CA）对油酸（OA）诱导的HepG2细胞脂质积累的影响。JBE和CA增加了OA处理的HepG2细胞中AMP激活的蛋白激酶（AMPK）、乙酰辅酶A羧化酶（ACC）的磷酸化以及肉碱棕榈酰转移酶1a（CPT1a）的表达。此外，这些化合物降低了固醇调节元件结合蛋白-1c（SREBP-1c）、脂肪酸合酶（FAS）以及OA诱导的脂质积累，表明JBE和CA通过AMPK激活途径调节HepG2细胞中的脂质代谢。
