Interdisciplinary Graduate Program in Advanced Convergence Technology & Science, Jeju National University, Jeju 63243, Republic of Korea.
Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Republic of Korea.
J Ethnopharmacol. 2018 Oct 5;224:335-348. doi: 10.1016/j.jep.2018.06.008. Epub 2018 Jun 12.
Sasa quelpaertensis Nakai is an edible dwarf bamboo cultivated mainly in Jeju Island, South Korea and its leaf displays various health-promoting properties including antioxidant scavenging.
In this study, we aimed at elucidating its hepatoprotective effect against alcohol-induced fatty liver.
In in vitro study, we evaluated the cytotoxicity and hepatoprotective effect of different solvent fractions (aqua, butanol, chloroform, ethyl acetate and hexane) of 80% EtOH extract of S. quelpaertensis Nakai leaf. In vivo experiment performed using binge alcohol consumption model.
Although all five fractions (0-1000 µg/mL) were non-cytotoxic to HepG2 cells, only ethyl acetate fraction (SQEA), rich in phenolic acids such as p-coumaric acid and flavonoids particularly myristin, showed hepatoprotective effect against EtOH (400 mM) in HepG2 cells. Furthermore, SQEA significantly decreased the ethanol induced cell death and enhanced the cell proliferation. In in vivo experiment using binge consumption model (5 g of EtOH/kg body weight in every 12 h for 3 times), SQEA treatment (10, 50 and 100 mg/kg) markedly reduced the alcohol induced histopathological changes and serum EtOH content, and reversed the reduction of glutathione level in ethanol challenged livers. Further, it suppressed the expression of cytochrome P450 2E1 (CYP2E1). In particular, SQEA activated AMP activated protein kinase (AMPK) pathway, and decreased the expression of tumor necrosis factor receptor-1 (TNFR1), which attenuated lipogenesis via decreased expression of fatty acid synthase (FAS). Inhibited lipogenesis due to SQEA treatment directed towards decreased perilipin-2 expression. These results indicate that SQEA has hypolipidemic effect which is mediated by decreased oxidative stress, increased fatty acid oxidation response and decreased lipogenesis.
Our results suggest the possibility of developing SQEA as a natural hepatoprotective agent potent in attenuating alcohol-induced fatty liver.
Sasa quelpaertensis Nakai 是一种可食用的矮竹，主要生长在韩国济州岛，其叶片具有多种促进健康的特性，包括抗氧化清除作用。
本研究旨在阐明其对酒精性脂肪肝的保肝作用。
在体外研究中，我们评估了 S. quelpaertensis Nakai 叶 80%乙醇提取物的不同溶剂部分（水、丁醇、氯仿、乙酸乙酯和正己烷）的细胞毒性和保肝作用。体内实验采用 binge 酒精消耗模型进行。
虽然所有五个部分（0-1000μg/ml）对 HepG2 细胞均无细胞毒性，但只有富含对香豆酸和类黄酮等酚酸的乙酸乙酯部分（SQEA）对 HepG2 细胞中的乙醇（400mM）具有保肝作用。此外，SQEA 显著降低了乙醇诱导的细胞死亡并增强了细胞增殖。在 binge 消耗模型（每 12 小时给予 5g/kg 体重的乙醇，共 3 次）的体内实验中，SQEA 处理（10、50 和 100mg/kg）显著减少了酒精引起的组织病理学变化和血清乙醇含量，并逆转了乙醇处理肝脏中谷胱甘肽水平的降低。此外，它抑制了细胞色素 P450 2E1（CYP2E1）的表达。特别是，SQEA 激活了 AMP 激活的蛋白激酶（AMPK）通路，并降低了肿瘤坏死因子受体-1（TNFR1）的表达，通过降低脂肪酸合酶（FAS）的表达来抑制脂肪生成。由于 SQEA 处理导致 perilipin-2 表达降低，脂肪生成受到抑制。这些结果表明，SQEA 具有降低血脂的作用，这是通过降低氧化应激、增加脂肪酸氧化反应和减少脂肪生成来介导的。
我们的结果表明，SQEA 有可能作为一种天然的保肝剂，用于减轻酒精性脂肪肝。
