Goshima Tetsuya, Tsuji Masaharu, Inoue Hiroyuki, Yano Shinichi, Hoshino Tamotsu, Matsushika Akinori
Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, Higashi-hiroshima, Hiroshima 739-0046, Japan.
Biosci Biotechnol Biochem. 2013;77(7):1505-10. doi: 10.1271/bbb.130173. Epub 2013 Jul 7.
The yeast Kluyveromyces marxianus is considered as a potential alternative to Saccharomyces cerevisiae in producing ethanol as a biofuel. In this study, we investigated the ethanol fermentation properties of novel K. marxianus strain DMB1, isolated from bagasse hydrolysates. This strain utilized sorbitol as well as various pentoses and hexoses as single carbon sources under aerobic conditions and produced ethanol from glucose in hydrolysates of the Japanese cedar at 42 °C. Reference strains K. marxianus NBRC1777 and S. cerevisiae BY4743 did not assimilate sorbitol or ferment lignocellulosic hydrolysates to ethanol at this temperature. Thus strain DMB1 appears to be optimal for producing bioethanol at high temperatures, and might provide a valuable means of increasing the efficiency of ethanol fermentation.
马克斯克鲁维酵母被认为是酿酒酵母在生产作为生物燃料的乙醇方面的一种潜在替代物。在本研究中，我们调查了从甘蔗渣水解物中分离出的新型马克斯克鲁维酵母菌株DMB1的乙醇发酵特性。该菌株在有氧条件下利用山梨醇以及各种戊糖和己糖作为单一碳源，并在42℃下从日本柳杉水解物中的葡萄糖生产乙醇。参考菌株马克斯克鲁维酵母NBRC1777和酿酒酵母BY4743在此温度下不能同化山梨醇或将木质纤维素水解物发酵成乙醇。因此，菌株DMB1似乎是高温生产生物乙醇的最佳选择，并且可能为提高乙醇发酵效率提供有价值的方法。
