Shintani M, Minaguchi K, Suzuki K, Lim K A
Department of Forensic Odontology, Tokyo Dental College, Chiba, Japan.
Biochem Genet. 1990 Apr;28(3-4):173-84. doi: 10.1007/BF00561335.
Three new variants of acidic proline-rich proteins (At, Au, Aw) were found in human parotid saliva by isoelectric focusing and basic gel electrophoresis. Electrophoretic comparison of the purified proteins and their tryptic peptides suggested minor charge and size differences from other acidic PRPs. Genetic and biochemical studies indicate that the At and Aw proteins are allelic products of the PRH1 locus. Gene frequencies of the At productive allele (PRH1(6)) in Japanese, Chinese, and Malays were 0.008, 0.012, and 0.004, respectively. The Au protein was also found in Japanese (2 in 746 samples), Chinese (1 in 215 samples), and Malays (1 in 220 samples), however, the Aw protein was found only in one Japanese (n = 746). These three proteins were not found in 106 Indian subjects.
通过等电聚焦和碱性凝胶电泳，在人腮腺唾液中发现了三种新的富含脯氨酸的酸性蛋白变体（At、Au、Aw）。对纯化蛋白及其胰蛋白酶肽段进行的电泳比较表明，它们与其他酸性富含脯氨酸蛋白在电荷和大小上存在细微差异。遗传和生化研究表明，At和Aw蛋白是PRH1基因座的等位基因产物。在日本人、中国人和马来人中，At有效等位基因（PRH1(6)）的基因频率分别为0.008、0.012和0.004。在日本人（746份样本中有2份）、中国人（215份样本中有1份）和马来人（220份样本中有1份）中也发现了Au蛋白，然而，Aw蛋白仅在一名日本人中发现（n = 746）。在106名印度受试者中未发现这三种蛋白。
