Dhawan A, Parmar D, Das M, Seth P K
Industrial Toxicology Research Centre, Lucknow, India.
Biochem Biophys Res Commun. 1990 Jul 31;170(2):441-7. doi: 10.1016/0006-291x(90)92111-c.
Distribution of the mixed function oxidases (MFO's) catalyzed by presence of multiple forms of cytochrome P-450 (P-450) was investigated in the neuronal and glial cells of the brain. The neuronal cells exhibited 2-3 fold higher activity of P-450 dependent arylhydrocarbon hydroxylase (AHH), 7-ethoxycoumarin-o-deethylase (ECOD) and 7-ethoxy-resorufn-o-deethylase (EROD) than the glial cells. Pretreatment with phenobarbital (PB) significantly increased (60-85%) the activity of ECOD in neuronal and glial cells, while a 140% increase was observed in neuronal AHH activity. Exposure to 3-methylcholanthrene (MC) resulted in a significant induction of the activity of AHH (102-345%), ECOD (115-150%) and EROD (75-120%) in the neuronal and glial cell preparations. The neurons, in general, exhibited greater sensitivity towards PB and MC induction. The present data indicate the differential sensitivity of these enzymes in neuronal and glial cells which could be used as a model to understand the selective action of certain neurotoxic agents.
在大脑的神经元和神经胶质细胞中，研究了由多种形式的细胞色素P - 450（P - 450）催化的混合功能氧化酶（MFO）的分布情况。神经元细胞中，细胞色素P - 450依赖的芳烃羟化酶（AHH）、7 - 乙氧基香豆素 - O - 脱乙基酶（ECOD）和7 - 乙氧基试卤灵 - O - 脱乙基酶（EROD）的活性比神经胶质细胞高2 - 3倍。用苯巴比妥（PB）预处理可使神经元和神经胶质细胞中ECOD的活性显著增加（60 - 85%），而神经元AHH活性增加了140%。暴露于3 - 甲基胆蒽（MC）导致神经元和神经胶质细胞制剂中AHH（102 - 345%）、ECOD（115 - 150%）和EROD（75 - 120%）的活性显著诱导。总体而言，神经元对PB和MC诱导表现出更高的敏感性。目前的数据表明这些酶在神经元和神经胶质细胞中的敏感性差异，这可作为理解某些神经毒性剂选择性作用的模型。
