School of Pharmacy, Showa University, Shinagawa-ku, Tokyo, 142-8555, Japan.
Luminescence. 2014 Jun;29(4):374-7. doi: 10.1002/bio.2555. Epub 2013 Jul 5.
We developed a highly sensitive chemiluminescent (CL) assay for hydrogen peroxide using 10-methyl-9-(phenoxycarbonyl) acridinium fluorosulfonate (PMAC) that produced chemiluminescence under neutral conditions and applied it to an enzyme immunoassay (EIA). One picomole of hydrogen peroxide could be detected using the optimized PMAC-CL method and 6.2 × 10(-20) mol β-D-galactosidase (β-gal) could be detected by combining an indoxyl derivative substrate and the proposed PMAC-CL method. This highly sensitive CL β-gal assay was applied to an EIA for thyroid-stimulating hormone (TSH) using β-gal as a label enzyme; 0.02-100.0 μU/mL TSH in human serum could be assayed directly and with high reproducibility.
我们开发了一种使用 10-甲基-9-(苯氧羰基)吖啶磺酸盐 (PMAC) 的高灵敏度化学发光 (CL) 测定法，用于过氧化氢，该方法在中性条件下产生化学发光，并将其应用于酶免疫分析 (EIA)。使用优化的 PMAC-CL 方法可以检测到 1 皮摩尔的过氧化氢，并且通过结合吲哚衍生物底物和建议的 PMAC-CL 方法，可以检测到 6.2×10(-20)molβ-D-半乳糖苷酶 (β-gal)。这种高灵敏度的 CLβ-gal 测定法应用于甲状腺刺激激素 (TSH) 的 EIA，使用β-gal 作为标记酶；可直接测定人血清中 0.02-100.0μU/mL 的 TSH，且具有高重现性。
