Bhattacharya Saurabh Kumar, Nathawat Lokendra Singh, Damani Priyankka, Choksi Arpan Kumar, Banik Arpita, Sinha Kriti, Bhattacharya Aditi Sarkar
Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University Vellore, Tamil Nadu, India.
Toxicol Int. 2013 Jan;20(1):11-8. doi: 10.4103/0971-6580.111535.
Wellbutrin (bupropion hydrochloride; WB), an anti-depressant of the aminoketone class is new highly selective norepinephrine and dopamine reuptake inhibitor; it is effective in the treatment of patients with major depression.
To investigate the in vitro effects of WB in human cultured peripheral blood lymphocytes and human cortical neural (HCN2) cell lines, micronucleus, sister chromatid exchange analysis, cellular viability, and comet assays were employed. The present study is to our knowledge, the first report on WB genotoxicity in cultured human peripheral blood lymphocytes and its cytotoxicity in the HCN2 cell line. We have also investigated the genotoxic potential of WB to induce chromosomal aberrations.
WB-induced cytotoxicity (measured as reduction of the nuclear division index) possibly prevented the division of damaged cells.
We conclude that although, WB exerts potential genotoxic effects in cultured lymphocytes, its cytogenetic effects are very unlikely to occur in blood cultures of WB-administered subjects.
安非他酮（盐酸安非他酮；WB）是一种氨基酮类抗抑郁药，是新型的高选择性去甲肾上腺素和多巴胺再摄取抑制剂；它对重度抑郁症患者的治疗有效。
为研究WB对人外周血淋巴细胞培养物和人皮质神经（HCN2）细胞系的体外作用，采用了微核试验、姐妹染色单体交换分析、细胞活力分析和彗星试验。据我们所知，本研究是关于WB对培养的人外周血淋巴细胞的遗传毒性及其对HCN2细胞系的细胞毒性的首次报道。我们还研究了WB诱导染色体畸变的遗传毒性潜力。
WB诱导的细胞毒性（以核分裂指数降低来衡量）可能阻止了受损细胞的分裂。
我们得出结论，虽然WB在培养的淋巴细胞中发挥潜在的遗传毒性作用，但其细胞遗传学效应在服用WB的受试者的血液培养物中极不可能发生。
