Department of Biochemistry and Molecular Biology, Bengbu Medical College, Anhui, 233030, China.
Curr Drug Targets. 2013 Sep;14(10):1150-6. doi: 10.2174/13894501113149990187.
Although genistein has been shown to inhibit tumorigenesis in a variety of human cancers including pancreatic cancer (PC), the exact molecular mechanism of its anti-cancer effects has not yet been fully elucidated. Recently, microRNAs (miRNAs) have been reported to regulate multiple aspects of tumor development and progression, indicating that targeting miRNAs could be a novel strategy to treat human cancers. In the current study, we investigated whether a natural compound genistein could down-regulate onco-miR-223, resulting in the inhibition of cell growth and invasion, and induction of apoptosis in PC cells. We found that genistein treatment significantly inhibited miR-223 expression and up-regulated Fbw7, one of the targets of miR-223. Moreover, down-regulation of miR-223 inhibited cell growth and induced apoptosis in PC cells. These findings suggest that genistein exerts its anti-tumor activity partly through downregulation of miR-223 in PC cells.
虽然染料木黄酮已被证明可抑制多种人类癌症（包括胰腺癌[PC]）的肿瘤发生，但它的抗癌作用的确切分子机制尚未完全阐明。最近，microRNAs（miRNAs）已被报道可调节肿瘤发生和进展的多个方面，表明靶向 miRNAs 可能是治疗人类癌症的一种新策略。在本研究中，我们研究了天然化合物染料木黄酮是否可以下调致癌 miRNA-223，从而抑制 PC 细胞的生长和侵袭，并诱导细胞凋亡。我们发现，染料木黄酮处理可显著抑制 miR-223 的表达，并上调 miR-223 的靶标之一 Fbw7。此外，下调 miR-223 可抑制 PC 细胞的生长并诱导细胞凋亡。这些发现表明，染料木黄酮通过下调 PC 细胞中的 miR-223 发挥其抗肿瘤活性。
