Goil M M
Z Parasitenkd. 1975 Jun 27;46(3):211-9. doi: 10.1007/BF00389879.
Extracts of adult Paramphistomum explanatum have been shown to contain high concentration of acid phosphomonoesterase with maximum activity at pH 4.5. The enzyme has been characterized by an exhibition of an unexpected increase in the inhibitory action of a mercury at 1 mM concentration by EDTA. With a lower concentration of mercury (0.1 mM and below) EDTA gave partial protection against inhibition. Different concentrations of magnesium and cobalt activated the enzyme while fluoride, copper, arsenate, tartrate and p-mercuribenzoate brought about inhibition. EDTA, glycine, glutathione and sodium azide had no effect. There was an indication of the presence of alkaline phosphomonoesterase at pH 10.0. The Km for p-nitrophenyl phosphate hydrolysis was 0.45 mM at pH 4.5.
已证明成年扁平阔盘吸虫的提取物含有高浓度的酸性磷酸单酯酶，在pH 4.5时活性最高。该酶的特征是在1 mM浓度下，汞的抑制作用会因乙二胺四乙酸（EDTA）而意外增加。在较低浓度的汞（0.1 mM及以下）时，EDTA可提供部分抗抑制保护。不同浓度的镁和钴可激活该酶，而氟化物、铜、砷酸盐、酒石酸盐和对汞苯甲酸会导致抑制。EDTA、甘氨酸、谷胱甘肽和叠氮化钠没有影响。在pH 10.0时有迹象表明存在碱性磷酸单酯酶。在pH 4.5时，对硝基苯磷酸酯水解的米氏常数（Km）为0.45 mM。
