Goil M M
Z Parasitenkd. 1978 Mar 16;55(1):59-62. doi: 10.1007/BF00383476.
Studies on aspartate aminotransferase (GOT) and L-alanine aminotransferase (GPT) of Paramphistomum explanatum have shown that GPT activity has more than twice the activity of GOT. The effect os some--SH reagents like cadmium, mercury, silver and iodoacetamide revealed that both enzymes were inhibited except that GOT was insensitive to cadmium ions. GPT was found to be much more sensitive to--SH reagents than GOT. There was unusual reaction to the two thiols used, cysteine and mercaptoethanol. Cysteine inhibited both the enzymes and mercaptoethanol activated GPT and inhibited GOT. Thiols in combination with iodoacetamide showed that the strong inhibitory effect of cysteine on both enzymes was reduced by iodoacetamide, but with mercaptoethanol the inhibitory effect on GOT was greater than when either of them was used alone, while GPT the effect of either counteracted each other. EDTA activated both enzymes and partially protected mercury inhibition of both enzymes and silver inhibition GOT only. It provided no protection against silver inhibition of GPT but complete protection of GPT against total inhibition by cadmium ions.
对透明双腔吸虫天冬氨酸转氨酶（GOT）和L-丙氨酸转氨酶（GPT）的研究表明，GPT活性是GOT活性的两倍多。镉、汞、银和碘乙酰胺等一些巯基试剂的作用表明，除了GOT对镉离子不敏感外，这两种酶均受到抑制。发现GPT比GOT对巯基试剂更敏感。对所使用的两种硫醇，即半胱氨酸和巯基乙醇有不同寻常的反应。半胱氨酸抑制这两种酶，而巯基乙醇激活GPT并抑制GOT。硫醇与碘乙酰胺联合使用表明，碘乙酰胺可降低半胱氨酸对这两种酶的强烈抑制作用，但对于巯基乙醇，其对GOT的抑制作用比对单独使用它们时更大，而对于GPT，两者的作用相互抵消。EDTA激活这两种酶，并部分保护它们免受汞的抑制以及仅GOT免受银的抑制。它不能保护GPT免受银的抑制，但能完全保护GPT免受镉离子的完全抑制。
