Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareli Road, Lucknow, 226 014, India.
Arch Virol. 2013 Dec;158(12):2561-75. doi: 10.1007/s00705-013-1783-7. Epub 2013 Jul 9.
The expression of matrix metalloproteinases (MMPs) is tightly regulated at the level of gene transcription, conversion of pro-enzyme to active MMPs, and the action of tissue inhibitors of metalloproteinases (TIMPs). The present study aimed to investigate the expression of some specific MMPs (2, 7, 9) and TIMPs (1, 2, 3) in serum and cerebrospinal fluid (CSF) of children with Japanese encephalitis virus (JEV) infection. Serum and CSF levels of MMPs and TIMPs in children with JEV infection and disease control (DC) were compared. The CSF and serum concentrations of MMP-2, TIMP-2 and TIMP-3 were significantly higher in children with JEV infection compared to DC. The concentration of MMP-9 in serum was significantly higher in children with JEV infection than in the DC and healthy control (HC), while in the CSF, no significant difference was observed compared to DC. The MMP-7 serum concentration was significantly higher in children with JEV infection compared to HC, but no significant difference was observed compared to DC. MMP-7 concentration was undetectable in CSF in both groups. The TIMP-1 CSF concentration was significantly higher, while the serum concentration was significantly lower, in children with JEV infection compared to DC. No correlation was found between the levels of each biomolecule measured in CSF and serum, suggesting that the levels in CSF represent local production within the CNS rather than production in the periphery. We also observed leucocytosis, mononuclear pleocytosis and elevated protein concentrations in the CSF of children with JEV infection compared to DC.
基质金属蛋白酶（MMPs）的表达在基因转录水平、前酶转化为活性 MMPs 以及金属蛋白酶组织抑制剂（TIMPs）的作用方面受到严格调控。本研究旨在探讨乙型脑炎病毒（JEV）感染患儿血清和脑脊液中某些特定 MMPs（2、7、9）和 TIMPs（1、2、3）的表达情况。比较了 JEV 感染患儿与疾病对照组（DC）的血清和脑脊液中 MMPs 和 TIMPs 的水平。与 DC 相比，JEV 感染患儿的脑脊液和血清中 MMP-2、TIMP-2 和 TIMP-3 的浓度明显升高。血清中 MMP-9 的浓度在 JEV 感染患儿中明显高于 DC 和健康对照组（HC），而脑脊液中与 DC 相比则无明显差异。与 HC 相比，JEV 感染患儿的血清 MMP-7 浓度明显升高，但与 DC 相比则无明显差异。两组 CSF 中均未检测到 MMP-7 浓度。与 DC 相比，JEV 感染患儿的脑脊液 TIMP-1 浓度明显升高，而血清浓度明显降低。在 CSF 和血清中测量的每种生物分子的水平之间未发现相关性，表明 CSF 中的水平代表 CNS 内的局部产生，而不是外周产生。与 DC 相比，我们还观察到 JEV 感染患儿的脑脊液中白细胞增多、单核细胞增多和蛋白浓度升高。
