对于育空河奇努克鲑鱼（Oncorhynchus tshawytscha）中霍氏鱼怪（Ichthyophonus hoferi）的诊断，聚合酶链式反应（PCR）检测的准确性可与培养法相媲美。

PCR testing can be as accurate as culture for diagnosis of Ichthyophonus hoferi in Yukon River Chinook salmon Oncorhynchus tshawytscha .

作者信息

Hamazaki Toshihide, Kahler Eryn, Borba Bonnie M, Burton Tamara

机构信息

Alaska Department of Fish and Game, Division of Commercial Fisheries, 333 Raspberry Road, Anchorage, Alaska 99518, USA.

出版信息

Dis Aquat Organ. 2013 Jul 9;105(1):21-5. doi: 10.3354/dao02606.

DOI:10.3354/dao02606

PMID:23836767

Abstract

We evaluated the comparability of culture and PCR tests for detecting Ichthyophonus in Yukon River Chinook salmon Oncorhynchus tshawytscha from field samples collected at 3 locations (Emmonak, Chena, and Salcha, Alaska, USA) in 2004, 2005, and 2006. Assuming diagnosis by culture as the 'true' infection status, we calculated the sensitivity (correctly identifying fish positive for Ichthyophonus), specificity (correctly identifying fish negative for Ichthyophonus), and accuracy (correctly identifying both positive and negative fish) of PCR. Regardless of sampling locations and years, sensitivity, specificity, and accuracy exceeded 90%. Estimates of infection prevalence by PCR were similar to those by culture, except for Salcha 2005, where prevalence by PCR was significantly higher than that by culture (p < 0.0001). These results show that the PCR test is comparable to the culture test for diagnosing Ichthyophonus infection.

摘要

我们评估了2004年、2005年和2006年在美国阿拉斯加3个地点（埃莫纳克、切纳和萨尔查）采集的育空河奇努克鲑鱼（Oncorhynchus tshawytscha）野外样本中，培养法和聚合酶链反应（PCR）检测鱼怪菌（Ichthyophonus）的可比性。假设以培养法诊断的结果为“真实”感染状况，我们计算了PCR的灵敏度（正确识别鱼怪菌阳性的鱼）、特异性（正确识别鱼怪菌阴性的鱼）和准确性（正确识别阳性和阴性的鱼）。无论采样地点和年份如何，灵敏度、特异性和准确性均超过90%。除2005年萨尔查地区外，PCR检测的感染率估计值与培养法相似，2005年萨尔查地区PCR检测的感染率显著高于培养法（p < 0.0001）。这些结果表明，PCR检测在诊断鱼怪菌感染方面与培养法具有可比性。