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用于诊断霍氏鱼醉菌的聚合酶链反应检测:对拉帕特拉和科坎(2013年)的回复

PCR testing for diagnosis of Ichthyophonus hoferi: reply to LaPatra & Kocan (2013).

作者信息

Hamazaki Toshihide, Kahler Eryn, Borba Bonnie M, Burton Tamara

机构信息

Alaska Department of Fish and Game, Division of Commercial Fisheries, 333 Raspberry Road, Anchorage, Alaska 99518, USA.

出版信息

Dis Aquat Organ. 2013 Nov 6;106(3):275-6. doi: 10.3354/dao02682.

Abstract

LaPatra & Kocan (2013) critiqued our paper Hamazaki et al. (2013; Dis Aquat Org 105:21-25) for data not supporting the conclusions of 'PCR testing is as accurate as culture…', but they neither pointed out what part of our data did not support our conclusion, nor did they provide any contrary scientific evidence supporting their argument that PCR testing is less accurate than culture. In the absence of any contradictory data, we stand by our data and our conclusion: PCR test is as suitable as culture as a diagnostic and field surveillance tool.

摘要

拉帕特拉和科坎(2013年)批评了我们的论文《滨崎等(2013年;《水生生物组织》105:21 - 25)》,称数据不支持“PCR检测与培养……一样准确”这一结论,但他们既未指出我们数据中的哪部分不支持我们的结论,也未提供任何相反的科学证据来支持他们关于PCR检测不如培养准确的论点。在没有任何矛盾数据的情况下,我们坚持我们的数据和结论:作为一种诊断和现场监测工具,PCR检测与培养一样适用。

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