Lin Wan-Chen, Yu Cheng-Han, Triffo Sara, Groves Jay T
Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, California.
Curr Protoc Chem Biol. 2010 Dec 1;2(4):235-69. doi: 10.1002/9780470559277.ch100131.
Supported membranes, formed as a single continuous lipid bilayer on a solid substrate, such as silica, have been used extensively as a model for protein-protein and cell-cell interaction, to study the molecular interactions at interfaces and the heterogeneities of plasma membranes. The advantages of a supported membrane system include the ability to control membrane composition and the compatibility it has with various surface-sensitive microscopic and spectroscopic techniques. Recent advances in micro- and nanotechnology have greatly extended the use of supported membranes to address key questions in cell biology. Although supported membranes can be easily made by vesicle fusion, the samples need careful preparation for this process to be efficient. The protocols in this unit comprehensively describe procedures to prepare, functionalize, and characterize supported membranes. Curr. Protoc. Chem. Biol. 2:235-269 © 2010 by John Wiley & Sons, Inc.
支撑膜在诸如二氧化硅等固体基质上形成为单一连续的脂质双层，已被广泛用作蛋白质 - 蛋白质和细胞 - 细胞相互作用的模型，以研究界面处的分子相互作用和质膜的异质性。支撑膜系统的优点包括能够控制膜的组成以及它与各种表面敏感的显微镜和光谱技术的兼容性。微纳技术的最新进展极大地扩展了支撑膜在解决细胞生物学关键问题方面的应用。尽管通过囊泡融合可以轻松制备支撑膜，但为了使该过程高效，样品需要仔细制备。本单元中的方案全面描述了制备、功能化和表征支撑膜的程序。《化学与生物学实验指南》第2卷：235 - 269页 2010年 约翰威立国际出版公司版权所有
