Ross-Rodriguez Lisa U, Elliott Janet A W, McGann Locksley E
Biopreserv Biobank. 2012 Oct;10(5):462-71. doi: 10.1089/bio.2012.0027.
Quantitative description of the non-ideal solution thermodynamics of the cytoplasm of a living mammalian cell is critically necessary in mathematical modeling of cryobiology and desiccation and other fields where the passive osmotic response of a cell plays a role. In the solution thermodynamics osmotic virial equation, the quadratic correction to the linear ideal, dilute solution theory is described by the second osmotic virial coefficient. Herein we report, for the first time, intracellular solution second osmotic virial coefficients for four cell types [TF-1 hematopoietic stem cells, human umbilical vein endothelial cells (HUVEC), porcine hepatocytes, and porcine chondrocytes] and further report second osmotic virial coefficients indistinguishable from zero (for the concentration range studied) for human hepatocytes and mouse oocytes.
对活的哺乳动物细胞胞质的非理想溶液热力学进行定量描述，对于低温生物学和干燥等领域的数学建模以及细胞被动渗透反应起作用的其他领域而言至关重要。在溶液热力学的渗透维里方程中，对线性理想稀溶液理论的二次校正由第二渗透维里系数描述。在此，我们首次报告了四种细胞类型[TF-1造血干细胞、人脐静脉内皮细胞（HUVEC）、猪肝细胞和猪软骨细胞]的细胞内溶液第二渗透维里系数，并进一步报告了人肝细胞和小鼠卵母细胞（在所研究的浓度范围内）的第二渗透维里系数与零无差异。
