Rodriguez-Prado Yahdira M, Kong Xiaoyuan, Fant Michael E
Department of Pediatrics, University of South Florida Morsani College of Medicine, Tampa, FL 33606, USA.
ISRN Obstet Gynecol. 2013 Jun 11;2013:704252. doi: 10.1155/2013/704252. Print 2013.
PLAC1 (Placenta-Specific 1) is a recently described, trophoblast-expressed gene essential for normal placental development. The protein localizes to the microvillus membrane surface of the syncytiotrophoblast in direct proximity to the maternal compartment. Although its role has not been defined, increased circulating levels of human PLAC1 mRNA in maternal blood are associated with preeclampsia. Furthermore, PLAC1-null mice exhibit decreased viability in the peripartum period suggesting a role in pregnancy maintenance late in gestation. We examined PLAC1 gene expression in the human placenta during normal pregnancy and pregnancies associated with maternal diabetes and preeclampsia using quantitative, real time PCR (q-RT-PCR). Although there was no apparent difference in PLAC1 gene expression among human pregnancies complicated by diabetes or preeclampsia, an unexpected effect of labor was noted at term. PLAC1 expression in placentae delivered vaginally following induced or spontaneous labor was significantly reduced compared to placentae not exposed to labor making it one of only a few placental genes influenced by labor. The significance of this finding is unknown. Viewed in the context of its importance in placental development, however, these findings are consistent with a role for PLAC1 in the maintenance of the maternal-fetal interface.
胎盘特异性蛋白1（PLAC1）是一种最近被描述的、由滋养层细胞表达的基因，对正常胎盘发育至关重要。该蛋白定位于合体滋养层细胞的微绒毛膜表面，紧邻母体腔室。尽管其作用尚未明确，但母体血液中人类PLAC1 mRNA循环水平的升高与子痫前期有关。此外，PLAC1基因敲除小鼠在围产期的生存能力下降，提示其在妊娠晚期维持妊娠中发挥作用。我们使用定量实时PCR（q-RT-PCR）检测了正常妊娠以及与母体糖尿病和子痫前期相关的妊娠中人类胎盘的PLAC1基因表达。尽管在合并糖尿病或子痫前期的人类妊娠中，PLAC1基因表达没有明显差异，但足月时发现了产程的意外影响。与未经历产程的胎盘相比，引产或自然分娩后经阴道娩出的胎盘的PLAC1表达显著降低，这使其成为少数受产程影响的胎盘基因之一。这一发现的意义尚不清楚。然而，从其在胎盘发育中的重要性来看，这些发现与PLAC1在维持母胎界面中的作用一致。
