新型抗生素组合抑制产超广谱β-内酰胺酶和金属β-内酰胺酶的体外研究：协同作用方法。
Novel combinations of antibiotics to inhibit extended-spectrum β-lactamase and metallo-β-lactamase producers in vitro: a synergistic approach.
机构信息
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
出版信息
Future Microbiol. 2013 Jul;8(7):939-44. doi: 10.2217/fmb.13.54.
AIM
To determine the synergistic effect of different antibiotics, alone and in combination, against extended-spectrum β-lactamase and metallo-β-lactamase producers.
MATERIALS & METHODS: MIC values of different antibiotics were determined for a total of 12 strains, of which nine strains were of clinical origin and the remainder were reference strains. In vitro synergy testing was performed for all the isolates using microdilution, chequerboard techniques and time-kill assays.
RESULTS
Out of the all the combinations, cefoxitin gave the highest rate of synergy by pairing up with streptomycin and cefotaxime to form effective combinations against multidrug-resistant strains of bacteria. However, the combination of cefotaxime and cefoxitin showed synergistic activity against both NDM-1-producing strains.
CONCLUSION
Novel synergistic combinations were observed against different resistant strains, including NDM-1 producers.
目的
确定不同抗生素单独及联合使用时对产超广谱β-内酰胺酶和金属β-内酰胺酶的协同作用。
材料与方法
对总共 12 株菌的不同抗生素的 MIC 值进行了测定，其中 9 株来自临床，其余为参考株。使用微量稀释法、棋盘技术和时间杀伤试验对所有分离株进行了体外协同试验。
结果
在所有组合中，头孢西丁与链霉素和头孢噻肟联合使用时协同作用最高，形成了针对多药耐药菌的有效组合。然而，头孢噻肟和头孢西丁的组合对 NDM-1 产生菌均表现出协同活性。
结论
针对不同的耐药株，包括 NDM-1 产生菌，观察到了新的协同组合。
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