Barbieri R, Giacomini P, Volinia S, Nastruzzi C, Mileo A M, Ferrini U, Soria M, Barrai I, Natali P G, Gambari R
Istituto di Chimica Biologica, Università di Ferrara, Italy.
FEBS Lett. 1990 Jul 30;268(1):51-4. doi: 10.1016/0014-5793(90)80970-t.
Synthetic oligonucleotides containing putative regulatory sequences are currently employed to identify and isolate genes coding for nuclear binding factors. Upstream DNA sequences of eukaryotic genes required for transcriptional activity and tissue specificity can be identified by means of biochemical techniques as well as computer analysis using homology searching. An alternative approach has been recently proposed by our research group. Scanning DNA sequences 1.8 megabases in length from a Genetic Sequence Data Bank, we have identified rare oligonucleotides 5 base pairs (bp) long, which are localized within or close to regulatory segments in mammalian promoters. In this paper we demonstrate that the rare GTATA sequence identifies an upstream region of the HLA-DR alpha gene which operates in conjunction with the sequence AGAAGTCAG, homologous to a box found in many interferon-inducible genes, in binding nuclear proteins.
目前，含有假定调控序列的合成寡核苷酸被用于鉴定和分离编码核结合因子的基因。转录活性和组织特异性所需的真核基因上游DNA序列可以通过生化技术以及使用同源性搜索的计算机分析来鉴定。我们的研究小组最近提出了另一种方法。通过扫描基因序列数据库中长度为1.8兆碱基的DNA序列，我们鉴定出了长度为5个碱基对（bp）的稀有寡核苷酸，它们位于哺乳动物启动子的调控区段内或附近。在本文中，我们证明了稀有GTATA序列可识别HLA - DRα基因的上游区域，该区域与序列AGAAGTCAG协同作用，AGAAGTCAG与许多干扰素诱导基因中发现的一个框同源，可结合核蛋白。
