Gambari R, Barbieri R, Nastruzzi C, Chiorboli V, Feriotto G, Natali P G, Giacomini P, Arcamone F
Istituto di Chimica Biologica, Università di Ferrara, Italy.
Biochem Pharmacol. 1991 Feb 15;41(4):497-502. doi: 10.1016/0006-2952(91)90620-k.
In this study we analyse the effects of the anti-tumor compound distamycin on the binding of nuclear factor(s) to a synthetic oligonucleotide (GTATA/IFN-gamma) mimicking a putative regulatory region of the human HLA-DR alpha gene. This region contains the sequence (GTATA), that is required for nuclear protein binding and is likely to interact with distamycin. The present results, by showing that distamycin inhibits the interaction between nuclear factors and the GTATA/IFN-gamma oligonucleotide, suggest that distamycin might alter the binding of transacting factors to cis-elements containing AT/TA sequences. Alterations of nuclear protein binding to specific target sequences could be one of the molecular mechanism(s) by which distamycin exerts its antiproliferative activity on living cells.
在本研究中，我们分析了抗肿瘤化合物偏端霉素对核因子与一种合成寡核苷酸（GTATA/IFN-γ）结合的影响，该寡核苷酸模拟了人类HLA-DRα基因的一个假定调控区域。该区域包含序列（GTATA），它是核蛋白结合所必需的，并且可能与偏端霉素相互作用。目前的结果表明，偏端霉素抑制核因子与GTATA/IFN-γ寡核苷酸之间的相互作用，这表明偏端霉素可能会改变反式作用因子与含有AT/TA序列的顺式元件的结合。核蛋白与特定靶序列结合的改变可能是偏端霉素对活细胞发挥其抗增殖活性的分子机制之一。
