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影响纵向小鼠 PET 研究中肿瘤(18)F-FDG 摄取的因素。

Factors affecting tumor (18) F-FDG uptake in longitudinal mouse PET studies.

机构信息

Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Rm. B2-085H CHS, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA.

Department of Radiation Oncology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.

出版信息

EJNMMI Res. 2013 Jul 10;3:51. doi: 10.1186/2191-219X-3-51. eCollection 2013.

Abstract

BACKGROUND

Many biological factors of 2-[(18) F]fluoro-2-deoxy-d-glucose ((18) F-FDG) in blood can affect (18) F-FDG uptake in tumors. In this study, longitudinal (18) F-FDG positron emission tomography (PET) studies were performed on tumor-bearing mice to investigate the effect of blood glucose level and tumor size on (18) F-FDG uptake in tumors.

METHODS

Six- to eight-week-old severe combined immunodeficiency mice were implanted with glioblastoma U87 (n = 8) or adenocarcinoma MDA-MB-231 (MDA) (n = 11) in the shoulder. When the tumor diameter was approximately 2.5 mm, a 60-min dynamic (18) F-FDG PET scan was performed weekly until the tumor diameter reached 10 mm. Regions of interests were defined in major organs and tumor. A plasma curve was derived based on a modeling method that utilizes the early heart time-activity curve and a late-time blood sample. The (18) F-FDG uptake constant K i was calculated using Patlak analysis on the tumors without an apparent necrotic center shown in the PET images. For each tumor type, the measured K i was corrected for partial volume (PV), and multivariate regression analysis was performed to examine the effects of blood glucose level ([Glc]) and tumor growth. Corrected Akaike's information criterion was used to determine the best model.

RESULTS

The regression model that best fit the PV-corrected K i for U87 data was K i /RC = (1/[Glc]) × (0.27 ± 0.027) mL/min/mL (where [Glc] is in mmol/L), and for MDA, it was K i /RC = (0.04 ± 0.005) mL/min/mL, where K i /RC denotes the PV-corrected K i using an individual recovery coefficient (RC). The results indicated that (18) F-FDG K i /RC for U87 was inversely related to [Glc], while [Glc] had no effect on (18) F-FDG K i /RC of MDA. After the effects of PV and [Glc] were accounted for, the data did not support any increase of (18) F-FDG K i as the tumor (of either type) grew larger in size.

CONCLUSIONS

The effect of [Glc] on the tumor (18) F-FDG K i was tumor-dependent. PV- and [Glc]-corrected (18) F-FDG K i did not show significant increase as the tumor of either type grew in size.

摘要

背景

血液中的 2-[(18)F]氟-2-脱氧-D-葡萄糖 ((18)F-FDG) 有许多生物学因素会影响肿瘤对 (18)F-FDG 的摄取。本研究通过对荷瘤小鼠进行纵向 (18)F-FDG 正电子发射断层扫描 (PET) 研究,探讨血糖水平和肿瘤大小对肿瘤中 (18)F-FDG 摄取的影响。

方法

将 6 至 8 周龄的严重联合免疫缺陷小鼠植入神经胶质瘤 U87(n=8)或腺癌 MDA-MB-231(MDA)(n=11)于肩部。当肿瘤直径约为 2.5 mm 时,每周进行 60 分钟的动态 (18)F-FDG PET 扫描,直到肿瘤直径达到 10 mm。在主要器官和肿瘤中定义了感兴趣区域。根据利用早期心脏时间-活性曲线和晚期血样的建模方法,得出血浆曲线。使用 Patlak 分析对 PET 图像中无明显坏死中心的肿瘤进行分析,计算 (18)F-FDG 摄取常数 K i。对于每种肿瘤类型,都对 K i 进行了部分容积 (PV)校正,并进行了多元回归分析,以检查血糖水平 ([Glc]) 和肿瘤生长的影响。使用校正的 Akaike 信息准则确定最佳模型。

结果

最适合 U87 数据的 PV 校正 K i 的回归模型为 K i /RC=(1/[Glc])×(0.27±0.027)mL/min/mL(其中 [Glc] 以 mmol/L 为单位),对于 MDA,它为 K i /RC=(0.04±0.005)mL/min/mL,其中 K i /RC 表示使用个体恢复系数 (RC) 的 PV 校正 K i。结果表明,U87 的 (18)F-FDG K i /RC 与 [Glc] 呈负相关,而 [Glc] 对 MDA 的 (18)F-FDG K i /RC 没有影响。在考虑了 PV 和 [Glc] 的影响后,数据不支持随着肿瘤(两种类型)的增大, (18)F-FDG K i 会有任何增加。

结论

[Glc] 对肿瘤 (18)F-FDG K i 的影响取决于肿瘤类型。经过 PV 和 [Glc] 校正后,随着肿瘤大小的增大,(18)F-FDG K i 没有显示出明显的增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b0/3718724/c906d9935cd3/2191-219X-3-51-1.jpg

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