Quirin Sean, Peterka Darcy S, Yuste Rafael
Howard Hughes Medical Institute, Department of Biological Sciences, Columbia University, 550 West 122nd Street, New York, NY 10027, USA.
Opt Express. 2013 Jul 1;21(13):16007-21. doi: 10.1364/OE.21.016007.
Imaging three-dimensional structures represents a major challenge for conventional microscopies. Here we describe a Spatial Light Modulator (SLM) microscope that can simultaneously address and image multiple targets in three dimensions. A wavefront coding element and computational image processing enables extended depth-of-field imaging. High-resolution, multi-site three-dimensional targeting and sensing is demonstrated in both transparent and scattering media over a depth range of 300-1,000 microns.
对传统显微镜而言,成像三维结构是一项重大挑战。在此,我们描述了一种空间光调制器(SLM)显微镜,它能够在三维空间中同时定位并成像多个目标。一个波前编码元件和计算图像处理实现了扩展景深成像。在300 - 1000微米的深度范围内,在透明和散射介质中均展示了高分辨率、多位点的三维靶向和传感。