Parshall C J, Vainisi S J, Goldberg M F, Wolf E D
Am J Vet Res. 1975 Jun;36(6):749-52.
The Japanese sika deep (Cervus nippon) proved to be a suitable animal model for the study of acute phases of in vivo erythrocyte sickling. Ophthalmologic studies can be conducted during or after 1 to 6 hours of effective in vivo sickling. Intravenous administration of 1.75 to 3.5% sodium bicarbonate solution at a rate of 500 to 1,000 ml per hour produced a transient state of alkalosis and in vivo erythrocyte sickling in the sika deer. The percentage of sickled erythrocytes increased as the blood pH increased. Concurrently, the packed cell volume decreased. Sickling was enhanced by 100% oxygen ventilation after endotracheal intubation and light anesthetization. After the induction of erythrocyte sickling, a sickling-reversal phenomenon occurred despite continued bicarbonate administration. During the course of this reversal, the percentage of sickled erythrocytes steadily decreased, the venous blood pH decreased, and the packed cell volume slowly increased. Because of the sickling-reversal phenomenon, chronic erythrocyte sickling was not achieved.
日本梅花鹿（Cervus nippon）被证明是研究体内红细胞镰变急性期的合适动物模型。可在体内有效镰变1至6小时期间或之后进行眼科研究。以每小时500至1000毫升的速度静脉注射1.75%至3.5%的碳酸氢钠溶液，会在梅花鹿体内产生短暂的碱中毒状态和红细胞镰变。镰状红细胞的百分比随着血液pH值的升高而增加。同时，血细胞比容降低。气管插管和轻度麻醉后，100%氧气通气可增强镰变。诱导红细胞镰变后，尽管持续给予碳酸氢盐，但仍出现了镰变逆转现象。在这种逆转过程中，镰状红细胞的百分比稳步下降，静脉血pH值下降，血细胞比容缓慢增加。由于镰变逆转现象，未能实现慢性红细胞镰变。
