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龙葵叶水提物诱导自噬并增强顺铂、阿霉素、多西他赛和 5-氟尿嘧啶对人结直肠癌细胞的细胞毒性。

Aqueous Extract of Solanum nigrum Leaves Induces Autophagy and Enhances Cytotoxicity of Cisplatin, Doxorubicin, Docetaxel, and 5-Fluorouracil in Human Colorectal Carcinoma Cells.

机构信息

Department of Chinese Medicine, Taipei Medical University Hospital, Taipei 11031, Taiwan ; Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.

出版信息

Evid Based Complement Alternat Med. 2013;2013:514719. doi: 10.1155/2013/514719. Epub 2013 Jun 17.

Abstract

Colorectal cancer is a common cancer worldwide, and chemotherapy is a mainstream approach for advanced and recurrent cases. Development of effective complementary drugs could help improve tumor suppression efficiency and control adverse effects from chemotherapy. The aqueous extract of Solanum nigrum leaves (AE-SN) is an essential component in many traditional Chinese medicine formulas for treating cancer, but there is a lack of evidence verifying its tumor suppression efficacy in colorectal cancer. The purpose of this study is to evaluate the tumor suppression efficacy of AE-SN using DLD-1 and HT-29 human colorectal carcinoma cells and examine the combined drug effect when combined with the chemotherapeutic drugs cisplatin, doxorubicin, docetaxel, and 5-fluorouracil. The results indicated that AE-SN induced autophagy via microtubule-associated protein 1 light chain 3 A/B II accumulation but not caspase-3-dependent apoptosis in both cell lines. The IC50s after 48 hours of treatment were 0.541 and 0.948 mg/ml AE-SN in DLD-1 and HT-29, respectively. AE-SN also demonstrated a combined drug effect with all tested drugs by enhancing cytotoxicity in tumor cells. Our results suggest that AE-SN has potential in the development of complementary chemotherapy for colorectal cancer.

摘要

结直肠癌是一种常见的癌症,在全球范围内,化疗是治疗晚期和复发性病例的主流方法。开发有效的辅助药物可以帮助提高肿瘤抑制效率,并控制化疗的不良反应。茄叶水提物(AE-SN)是许多治疗癌症的中药配方中的重要组成部分,但缺乏其在结直肠癌中抑制肿瘤的功效的证据。本研究旨在使用 DLD-1 和 HT-29 人结肠癌细胞评估 AE-SN 的肿瘤抑制功效,并研究其与顺铂、阿霉素、多西他赛和 5-氟尿嘧啶等化疗药物联合用药的效果。结果表明,AE-SN 在两种细胞系中通过微管相关蛋白 1 轻链 3 A/B II 的积累而非 caspase-3 依赖性细胞凋亡诱导自噬。在 48 小时处理后,DLD-1 和 HT-29 细胞中 AE-SN 的 IC50 值分别为 0.541 和 0.948 mg/ml。AE-SN 还通过增强肿瘤细胞的细胞毒性与所有测试药物显示出联合药物作用。我们的结果表明,AE-SN 具有开发结直肠癌辅助化疗的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf09/3703357/0b5d03443bb3/ECAM2013-514719.001.jpg

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