鸡疱疹病毒 2 诱导的鸡淋巴瘤中的微卫星不稳定性。

Microsatellite instability in chicken lymphoma induced by gallid herpesvirus 2.

机构信息

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.

出版信息

PLoS One. 2013 Jul 2;8(7):e68058. doi: 10.1371/journal.pone.0068058. Print 2013.

DOI:10.1371/journal.pone.0068058

PMID:23844155

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3699484/

Abstract

Microsatellite instability (MSI) has been found in a range of human tumors, and little is known of the links between MSI and herpesvirus. In order to investigate the relationship between MSI and Gallid herpesvirus 2 (GaHV-2)-induced lymphoma, fifteen Marek's disease (MD) lymphomas were analyzed through using 46 microsatellite markers, which were amplified by PCR from DNA specimens of lymphoma and normal muscular tissues from the same chicken. PCR products were evaluated by denaturing polyacrylamide gel electrophoresis for MSI analysis. MSI was proved in all lymphomas, at least in one locus. Thirty of the 46 microsatellite markers had microsatellite alterations. These results suggested that GaHV-2-induced lymphoma in chickens is related to MSI, and this is the first report to demonstrate that MSI is associated with the GaHV-2 induced lymphoma in chicken.

摘要

微卫星不稳定性（MSI）已在多种人类肿瘤中被发现，但 MSI 与疱疹病毒之间的联系知之甚少。为了研究 MSI 与禽疱疹病毒 2（GaHV-2）诱导的淋巴瘤之间的关系，使用 46 个微卫星标记物对 15 例马立克氏病（MD）淋巴瘤进行了分析，这些标记物通过 PCR 从同一只鸡的淋巴瘤和正常肌肉组织的 DNA 标本中扩增而来。通过变性聚丙烯酰胺凝胶电泳对 PCR 产物进行 MSI 分析。所有淋巴瘤均存在 MSI，至少在一个位点存在 MSI。在 46 个微卫星标记物中有 30 个发生了微卫星改变。这些结果表明，鸡的 GaHV-2 诱导的淋巴瘤与 MSI 有关，这是首次证明 MSI 与鸡的 GaHV-2 诱导的淋巴瘤有关。