a Departamentos de Industrias y Química Orgánica , Facultad de Ciencias Exactas y Naturales (FCEN), Universidad de Buenos Aires (UBA) , Buenos Aires , Argentina.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2013;30(9):1573-80. doi: 10.1080/19440049.2013.810347. Epub 2013 Jul 12.
The aim of this study was to quantify polycyclic aromatic hydrocarbon (PAH) levels in milk powder samples commercialised in Argentina and Brazil during 2012. Thirty-one samples were available from the retail market. An HPLC method for the determination of PAHs was applied involving a clean-up step with silica cartridges. Recoveries were greater than 79% for all PAHs analysed. Reproducible determination with adequate detection and quantification limits (LOD and LOQ) were attained by HPLC with fluorescence detection for 14 PAHs. Acenaphthylene was determined with a UV-VIS detector. There is no significant difference in any PAHs or in the sum of them between the Argentinean and Brazilian samples. Therefore, the samples were evaluated together. The highest concentration of benzo(a)pyrene (BaP) detected was 0.57 µg kg⁻¹ in milk powder. Contamination of samples expressed as the sum of 15 analysed PAHs varied between 11.8 and 78.4 µg kg⁻¹ and as PAH4 (BaP, chrysene, benzo(a)anthracene and benzo(b)fluoranthene) was between 0.02 and 10.16 µg kg⁻¹. The correlation coefficient for PAH2 (BaP and chrysene) and PAH4 groups was 0.95, for PAH2 and PAH8 it was 0.71, and for PAH4 and PAH8 it was 0.83. All the samples were below the regulatory limit for BaP, but 65% of commercial milk powders do not comply with the European Union limit for PAH4. This is the first report of PAH contamination in powder milk from Argentina and Brazil.
本研究旨在量化 2012 年在阿根廷和巴西商业化销售的奶粉样本中的多环芳烃（PAH）水平。从零售市场获得了 31 个样本。应用高效液相色谱法（HPLC）测定 PAHs，涉及硅胶柱的净化步骤。所有分析的 PAHs 的回收率均大于 79%。通过 HPLC 与荧光检测，可实现 14 种 PAHs 的重现性测定，并具有足够的检测限（LOD）和定量限（LOQ）。苊烯用紫外可见检测器测定。阿根廷和巴西样本中任何一种 PAHs 或它们总和之间没有显著差异。因此，将样本一起评估。在奶粉中检测到的最高浓度的苯并(a)芘（BaP）为 0.57μg/kg。以 15 种分析的 PAHs 总和表示的样品污染量在 11.8 和 78.4μg/kg 之间，以 PAH4（BaP、荧蒽、苯并(a)蒽和苯并(b)荧蒽）表示的污染量在 0.02 和 10.16μg/kg 之间。PAH2（BaP 和荧蒽）和 PAH4 组的相关系数为 0.95，PAH2 和 PAH8 组的相关系数为 0.71，PAH4 和 PAH8 组的相关系数为 0.83。所有样品均低于 BaP 的监管限值，但 65%的商业奶粉不符合欧盟对 PAH4 的限值要求。这是阿根廷和巴西奶粉中 PAH 污染的首次报告。
