Battisti Chiara, Girelli Anna Maria, Tarola Anna Maria
a Department of Chemistry , Sapienza University of Rome , Rome , Italy.
Food Addit Contam Part B Surveill. 2015;8(1):50-5. doi: 10.1080/19393210.2014.968880. Epub 2014 Dec 15.
The concentrations and distributions of major polycyclic aromatic hydrocarbons (PAHs) were determined in 20 kinds of yogurt specimens collected from Italian supermarkets using reversed phase high-performance liquid chromatography equipped with fluorescence detection. The method was validated by determination of recovery percentages, precision (repeatability) and sensitivity (limits of detection) with yogurt samples fortified at 0.25, 0.5 and 1 µg/kg concentration levels. The recovery of 13 PAHs, with the exception of naphthalene and acenaphthene, ranged from 61% to 130% and from 60% to 97% at all the levels for yogurts with low (0.1%) and high (3.9%) fat content, respectively. The method is repeatable with relative standard deviation values <20% for all analytes. The results obtained demonstrate that acenaphthene, fluorantene, phenanthrene, anthracene, fluoranthene and pyrene were found in all samples with a similar distribution, but different content when yogurts with low and high fats were compared.
使用配备荧光检测的反相高效液相色谱法，测定了从意大利超市采集的20种酸奶样本中主要多环芳烃（PAHs）的浓度和分布情况。通过对浓度水平为0.25、0.5和1 μg/kg的加标酸奶样本进行回收率、精密度（重复性）和灵敏度（检测限）测定，对该方法进行了验证。除萘和苊烯外，13种PAHs在低脂肪（0.1%）和高脂肪（3.9%）酸奶中的回收率在所有添加水平下分别为61%至130%和60%至97%。该方法对于所有分析物的相对标准偏差值<20%，具有可重复性。所得结果表明，所有样本中均检测到苊烯、荧蒽、菲、蒽、荧蒽和芘，它们的分布相似，但在比较低脂和高脂酸奶时含量不同。
