State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330031, China.
Anal Chim Acta. 2013 Jul 25;788:24-31. doi: 10.1016/j.aca.2013.06.016. Epub 2013 Jun 20.
Hydrogen peroxide is an important analyte in biochemical, industrial and environmental systems. Therefore, development of novel rapid and sensitive analytical methods is useful. In this work, a hemin-graphene nano-sheets (H-GNs)/gold nano-particles (AuNPs) electrochemical biosensor for the detection of hydrogen peroxide (H2O2) was researched and developed; it was constructed by consecutive, selective modification of the GCE electrode. Performance of the H-GNs/AuNPs/GCE was investigated by chronoamperometry, and AFM measurements suggested that the graphene flakes thickness was ~1.3 nm and that of H-GNs was ~1.8 nm, which ultimately indicated that each hemin layer was ~0.25 nm. This biosensor exhibited significantly better electrocatalytic activity for the reduction of hydrogen peroxide in comparison with the simpler AuNPs/GCE and H-GNs/GCE; it also displayed a linear response for the reduction of H2O2 in the range of 0.3 μM to 1.8 mM with a detection limit of 0.11μM (SN(-1)=3), high sensitivity of 2774.8 μA mM(-1) cm(-2), and a rapid response, which reached 95% of the steady state condition within 5s. In addition, the biosensor was unaffected by many interfering substances, and was stable over time. Thus, it was demonstrated that this biosensor was potentially suitable for H2O2 analysis in many types of sample.
过氧化氢是生化、工业和环境系统中的重要分析物。因此，开发新型快速灵敏的分析方法是有用的。在这项工作中，研究和开发了一种基于血红素-石墨烯纳米片（H-GNs）/金纳米粒子（AuNPs）的电化学生物传感器，用于检测过氧化氢（H2O2）；它是通过连续、选择性地修饰 GCE 电极构建的。通过计时安培法研究了 H-GNs/AuNPs/GCE 的性能，AFM 测量表明石墨烯片的厚度约为 1.3nm，H-GNs 的厚度约为 1.8nm，这最终表明每个血红素层约为 0.25nm。与简单的 AuNPs/GCE 和 H-GNs/GCE 相比，该生物传感器对过氧化氢的还原表现出明显更好的电催化活性；它还显示出在 0.3μM 至 1.8mM 的范围内对 H2O2 的还原具有线性响应，检测限为 0.11μM（SN(-1)=3），高灵敏度为 2774.8μA mM(-1) cm(-2)，并且响应迅速，在 5s 内达到稳态的 95%。此外，该生物传感器不受许多干扰物质的影响，并且随时间稳定。因此，证明该生物传感器适用于许多类型样品中的 H2O2 分析。
