School of Life and Environment Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.
School of Life and Environment Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China; National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Nanning, Guangxi 530021, China.
Biosens Bioelectron. 2016 Aug 15;82:185-94. doi: 10.1016/j.bios.2016.04.004. Epub 2016 Apr 8.
A highly sensitive non-enzymatic electrochemical sensor based on platinum nanoparticles/reduced graphene oxide-chitosan-ferrocene carboxylic acid nano-hybrids (Pt NPs/RGO-CS-Fc biosensor) was developed for the measurement of hydrogen peroxide (H2O2). The RGO-CS-Fc nano-hybrids was prepared and characterized by UV-vis spectrum, Fourier transform infrared spectroscopy, transmission electron microscopy, Raman spectrometer and electrochemical impedance spectroscopy. Under optimal experimental conditions, the Pt NPs/RGO-CS-Fc biosensor showed outstanding catalytic activity toward H2O2 reduction. The current response of the biosensor presented a linear relationship with H2O2 concentration from 2.0×10(-8)M to 3.0×10(-6)M with a correlation coefficient of R(2)=0.9968 and with logarithm of H2O2 concentration from 6.0×10(-6)M to 1.0×10(-2)M with a correlation coefficient of R(2)=0.9887, the low detection limit of 20nM was obtained at the signal/noise (S/N) ratio of 3. Moreover, the Pt NPs/RGO-CS-Fc biosensor exhibited excellent anti-interference capability and reproducibility for the detection of H2O2. The biosensor was also successfully applied for the detection of H2O2 from living cells containing normal and cancer cells. All these results prove that the Pt NPs/RGO-CS-Fc biosensor has the potential application in clinical diagnostics to evaluate oxidative stress of different living cells.
基于铂纳米粒子/还原氧化石墨烯-壳聚糖-二茂铁羧酸纳米杂化物（Pt NPs/RGO-CS-Fc 生物传感器）的高灵敏度非酶电化学传感器被开发用于测量过氧化氢（H2O2）。通过紫外-可见光谱、傅里叶变换红外光谱、透射电子显微镜、拉曼光谱和电化学阻抗谱对 RGO-CS-Fc 纳米杂化物进行了制备和表征。在最佳实验条件下，Pt NPs/RGO-CS-Fc 生物传感器对 H2O2 还原表现出出色的催化活性。该生物传感器的电流响应与 H2O2 浓度在 2.0×10(-8)M 至 3.0×10(-6)M 之间呈线性关系，相关系数 R(2)=0.9968，与 H2O2 浓度的对数在 6.0×10(-6)M 至 1.0×10(-2)M 之间呈线性关系，相关系数 R(2)=0.9887，检测限低至 20nM（信号/噪声比为 3）。此外，Pt NPs/RGO-CS-Fc 生物传感器在检测 H2O2 时表现出出色的抗干扰能力和重现性。该生物传感器还成功应用于含有正常和癌细胞的活细胞中 H2O2 的检测。所有这些结果都证明，Pt NPs/RGO-CS-Fc 生物传感器具有在临床诊断中评估不同活细胞氧化应激的潜在应用。
