Deutsch J, Soncrant T T, Greig N H, Rapoport S I
Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892.
J Chromatogr. 1990 Jun 29;528(2):325-31. doi: 10.1016/s0378-4347(00)82391-9.
Scopolamine, a muscarinic receptor antagonist, was measured in rat plasma and brain using gas chromatography-mass spectrometry with electron-impact ionization detection. Extracted scopolamine was either directly derivatized or first hydrolyzed to scopine, then derivatized with heptafluorobutyric anhydride, separated on a capillary column and quantified by mass fragmentography. Electron-impact ionization produced a common fragment peak at m/z 138 that was monitored along with trideuterated scopolamine, an internal standard (m/z 141). The method can be used to measure scopolamine concentrations of 2 ng/ml in rat plasma and 20 ng/g in rat brain.
使用带有电子轰击电离检测的气相色谱 - 质谱联用技术测定大鼠血浆和大脑中的毒蕈碱受体拮抗剂东莨菪碱。提取的东莨菪碱要么直接衍生化，要么先水解为东莨菪醇，然后用七氟丁酸酐衍生化，在毛细管柱上分离并通过质量碎片分析法进行定量。电子轰击电离在m/z 138处产生一个共同的碎片峰，与作为内标物的氘代东莨菪碱（m/z 141）一起进行监测。该方法可用于测定大鼠血浆中东莨菪碱浓度为2 ng/ml，大鼠大脑中为20 ng/g。
