Bayne W F, Tao F T, Crisologo N
J Pharm Sci. 1975 Feb;64(2):288-91. doi: 10.1002/jps.2600640220.
A GLC-mass spectrometric method for scopolamine, sensitive to 50 pg/ml for a 4-ml plasma or urine sample, was developed. The method used a deuterated internal standard to minimize variability in absolute recovery in the extraction procedure. Scopoline and deuterated scopoline were formed from the base-catalyzed hydrolysis of scopolamine and the internal standard and were analyzed as the heptafluorobutyrates, using a GLC-mass spectrometric system by monitoring the m/e 138 and 141 fragments, respectively.
已开发出一种用于东莨菪碱的气相色谱-质谱法，对于4毫升血浆或尿液样本，其检测灵敏度为50皮克/毫升。该方法使用氘代内标以最小化萃取过程中绝对回收率的变异性。东莨菪碱和内标经碱催化水解生成东莨菪醇和氘代东莨菪醇，并以七氟丁酸酯形式进行分析，通过气相色谱-质谱系统分别监测m/e 138和141碎片。
