热解气相色谱-质谱联用同时测定脑中乙酰胆碱和胆碱

Simultaneous measurement of acetylcholine and choline in brain by pyrolysis-gas chromatography-mass spectrometry.

作者信息

Khandelwal J K, Szilagyi P I, Barker L A, Green J P

出版信息

Eur J Pharmacol. 1981 Dec 3;76(2-3):145-56. doi: 10.1016/0014-2999(81)90496-9.

Abstract

Pyrolysis-gas chromatography and chemical ionization mass fragmentography were combined to develop a specific, simple and rapid method for simultaneously measuring endogenous and stable isotopic variants of acetylcholine and choline with a detection limit of approximately 10(-12) mol. The recovery and reproducibility of the method are excellent, and the method is suitable for measuring acetylcholine and choline in discrete regions of rat brain and to measure incorporation of choline into acetylcholine, both of which uses are demonstrated. This method affords easy analysis of 40 samples in a working day. The new technique used to extract compounds from tissues and the modified gas flow arrangement may be useful to measure other compounds as well.

摘要

热解气相色谱法和化学电离质谱碎片分析法相结合，开发出一种特异、简单且快速的方法，用于同时测量乙酰胆碱和胆碱的内源性及稳定同位素变体，检测限约为10⁻¹²摩尔。该方法的回收率和重现性极佳，适用于测量大鼠脑离散区域中的乙酰胆碱和胆碱，以及测量胆碱掺入乙酰胆碱的情况，这两种用途均得到了验证。此方法在一个工作日内可轻松分析40个样品。用于从组织中提取化合物的新技术以及改进的气流配置可能也有助于测量其他化合物。