Craig R G, Hanks C T
School of Dentistry, University of Michigan, Ann Arbor 48109-1078.
J Dent Res. 1990 Aug;69(8):1539-42. doi: 10.1177/00220345900690081801.
The cytotoxicity of a series of 29 experimental alloys and six pure metals was determined with cell culture techniques and succinic dehydrogenase histochemistry. The width of any ring of inhibition, optical density of the histochemically stained cells, and a visual ranking of the intensity of the blue color of the stained cells were compared for determination of cytotoxicity. Twenty-four of the 35 metals and alloys (approximately 70%) had the same rankings by the three methods. Of the pure metals, Au, Pd, and Ti were the least cytotoxic, followed by Ag, then Ni, and finally, Cu. Single-phase alloys with moderately high Cu and without high Pd and Au concentrations had high cytotoxicity, as did multiphase alloys, even when they were high in Au and Ag. High Pd was more effective in maintaining the biocompatibility of alloys containing Cu than was Au. Single-phase alloys with compositions typical of those to be used for porcelain-fused-to-metal restorations showed good biocompatibility, as did those base metal alloys that formed adherent oxide surface layers.
采用细胞培养技术和琥珀酸脱氢酶组织化学方法，测定了29种实验合金和6种纯金属的细胞毒性。比较了任何抑制环的宽度、组织化学染色细胞的光密度以及染色细胞蓝色强度的视觉分级，以确定细胞毒性。35种金属和合金中的24种（约70%）通过这三种方法得到的排名相同。在纯金属中，金、钯和钛的细胞毒性最小，其次是银，然后是镍，最后是铜。铜含量中等且不含高浓度钯和金的单相合金具有高细胞毒性，多相合金也是如此，即使它们富含金和银。高钯在维持含铜合金的生物相容性方面比金更有效。具有典型烤瓷熔附金属修复体成分的单相合金显示出良好的生物相容性，形成附着氧化表面层的贱金属合金也是如此。
