Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.
J Adv Prosthodont. 2013 Aug;5(3):341-50. doi: 10.4047/jap.2013.5.3.341. Epub 2013 Aug 31.
To evaluate adherence of human gingival fibroblasts (HGFs) to transmucosal abutment of dental implant with different surface conditions with time and to investigate the roles of focal adhesion linker proteins (FALPs) involved in HGFs adhesion to abutment surfaces.
Morphologies of cultured HGFs on titanium and ceramic discs with different surface were observed by scanning electron microscopy. Biocompatibility and focal adhesion were evaluated by ultrasonic wave application and cell viability assay. FALPs expression levels were assessed by RT-PCR and western blot.
There seemed to be little difference in biocompatibility and adhesion strength of HGFs depending on the surface conditions and materials. In all experimental groups, the number of cells remaining on the disc surface after ultrasonic wave application increased more than 2 times at 3 days after seeding compared to 1-day cultured cells and this continued until 7 days of culture. FALPs expression levels, especially of vinculin and paxillin, also increased in 5-day cultured cells compared to 1-day cultured fibroblasts on the disc surface.
These results might suggest that the strength of adhesion of fibroblasts to transmucosal abutment surfaces increases with time and it seemed to be related to expressions of FALPs.
评估不同表面条件下的黏膜下种植体穿黏膜基台与人类牙龈成纤维细胞（HGF）之间随时间的黏附情况，并研究参与 HGF 黏附到基台表面的黏着斑连接蛋白（FALP）的作用。
通过扫描电子显微镜观察培养在不同表面的钛和陶瓷片上的 HGF 的形态。通过超声应用和细胞活力测定评估生物相容性和黏附。通过 RT-PCR 和 Western blot 评估 FALP 表达水平。
HGF 的生物相容性和黏附强度似乎与表面条件和材料关系不大。在所有实验组中，与培养 1 天的细胞相比，在接种后 3 天，留在盘表面的细胞数量在超声处理后增加了 2 倍以上，这种情况一直持续到培养 7 天。与培养 1 天的盘表面成纤维细胞相比，黏着斑连接蛋白（特别是 vinculin 和 paxillin）的表达水平在培养 5 天的细胞中也增加了。
这些结果表明，成纤维细胞与黏膜下基台表面的黏附强度随时间增加，并且似乎与 FALP 的表达有关。
