Schroeckenstein D C, Meier-Davis S, Yunginger J W, Bush R K
Allergy Section, William S. Middleton Veterans Administration Hospital, Madison, WI 53705.
J Allergy Clin Immunol. 1990 Aug;86(2):189-93. doi: 10.1016/s0091-6749(05)80065-x.
Although the floral industry deals with many potential allergens, few examples of occupational asthma exist in this industry. A 22-year-old florist experienced symptoms of rhinitis, conjunctivitis, and asthma on exposure to baby's breath. To determine the contribution of baby's breath to the patient's symptoms, an extract of baby's breath was prepared. Prick skin tests with a 1:10(-5) wt/vol concentration of the extract produced an immediate response, whereas nonexposed atopic and normal control subjects did not react. The patient's asthmatic response to baby's breath was confirmed by bronchial challenge that caused an immediate fall in FEV1 of 26.2% from baseline after inhalation of 88 breath units of the extract. With a direct RAST, the patient's serum bound 38 times the amount of IgE bound by the negative control. IgE binding in the RAST was inhibited by the baby's breath extract but not by unrelated inhibitors (ragweed and tree pollens). Immunoblotting demonstrated IgE binding to 13 protein bands in the extract with molecular weights ranging from 11.5 to 68 kd. Serum from a patient previously reported to have sensitivity to baby's breath recognized five protein bands. Three proteins with molecular weights of 27, 31, and 37 kd were recognized by both patients' sera. We conclude that this patient developed IgE-mediated sensitivity to multiple allergens in baby's breath. This study confirms the importance of this plant as a potential cause of occupational asthma in the floral industry.
尽管花卉行业涉及许多潜在的过敏原，但该行业中职业性哮喘的案例却很少。一名22岁的花店店员在接触满天星时出现了鼻炎、结膜炎和哮喘症状。为了确定满天星对患者症状的影响，制备了满天星提取物。用1:10(-5)重量/体积浓度的提取物进行点刺皮肤试验产生了即时反应，而未接触过的特应性和正常对照受试者没有反应。吸入88个呼吸单位的提取物后，支气管激发试验导致患者的FEV1从基线立即下降26.2%，从而证实了患者对满天星的哮喘反应。通过直接RAST检测，患者血清结合的IgE量是阴性对照的38倍。RAST中的IgE结合被满天星提取物抑制，但未被无关抑制剂（豚草和树花粉）抑制。免疫印迹显示IgE与提取物中分子量范围为11.5至68 kd的13条蛋白带结合。一名先前报告对满天星敏感的患者的血清识别出五条蛋白带。两名患者的血清均识别出分子量为27、31和37 kd的三种蛋白质。我们得出结论，该患者对满天星中的多种过敏原产生了IgE介导的敏感性。这项研究证实了这种植物作为花卉行业职业性哮喘潜在病因的重要性。
