Lopez-Rubio A, Rodriguez J, Crespo J F, Vives R, Daroca P, Reaño M
Servicio de Alergia, Hospital Universitario 12 de Octubre, Madrid, Spain.
Allergy. 1998 Dec;53(12):1216-20. doi: 10.1111/j.1398-9995.1998.tb03845.x.
Vegetables of the Liliaceae family, such as garlic or onion, have been reported to cause occupational asthma. However, there are few data on adverse reactions to asparagus. We evaluated the role of asparagus as a cause of asthma in a patient with respiratory symptoms occurring at work (horticulture) and studied relevant allergens.
A 28-year-old man complained of rhinoconjunctivitis and asthma when harvesting asparagus at work. Eating cooked asparagus did not provoke symptoms. A positive skin test reaction was observed with raw asparagus, Alternaria alternata, and grass-pollen extracts. The methacholine test demonstrated mild bronchial hyperresponsiveness. The patient had an immediate asthmatic response after challenge with raw asparagus extract. Bronchial provocation tests with boiled asparagus, A. alternata, and control extracts were negative. Two unexposed subjects with seasonal allergic asthma did not react to the raw asparagus extract.
The double-blind, placebo-controlled food challenge with raw asparagus was negative. Serum asparagus-specific IgE was 13.9 kU(A)/l. By SDS-PAGE immunoblot, at least six IgE-binding components, ranging from 22 to 73 kDa, were detected only in raw asparagus.
We report a case of occupational asthma caused by asparagus inhalation, confirmed by specific bronchoprovocation. Immunoblot analysis showed that asparagus allergens are very labile and quite sensitive to heat denaturation.
据报道，百合科蔬菜，如大蒜或洋葱，可导致职业性哮喘。然而，关于芦笋不良反应的数据很少。我们评估了芦笋在一名工作（园艺）时出现呼吸道症状的患者中引发哮喘的作用，并研究了相关过敏原。
一名28岁男性在工作中收割芦笋时出现鼻结膜炎和哮喘症状。食用煮熟的芦笋不会引发症状。对生芦笋、链格孢菌和草花粉提取物进行皮肤试验，结果呈阳性。乙酰甲胆碱试验显示轻度支气管高反应性。用生芦笋提取物激发后，患者出现即刻哮喘反应。用煮熟的芦笋、链格孢菌和对照提取物进行支气管激发试验均为阴性。两名未接触过过敏原的季节性过敏性哮喘患者对生芦笋提取物无反应。
用生芦笋进行的双盲、安慰剂对照食物激发试验为阴性。血清芦笋特异性IgE为13.9 kU(A)/l。通过SDS-PAGE免疫印迹法，仅在生芦笋中检测到至少六种IgE结合成分，分子量范围为22至73 kDa。
我们报告了一例因吸入芦笋导致职业性哮喘的病例，经特异性支气管激发试验证实。免疫印迹分析表明，芦笋过敏原非常不稳定，对热变性相当敏感。
