Mattsby-Baltzer I, Edebo L, Järvholm B, Lavenius B, Söderström T
Department of Clinical Bacteriology, University of Göteborg, Sweden.
J Allergy Clin Immunol. 1990 Aug;86(2):231-8. doi: 10.1016/s0091-6749(05)80070-3.
The subclass distribution of the IgG and IgA antibody response in serum was studied in humans exposed to aerosolized metal-working fluid containing Pseudomonas pseudoalcaligenes. This species was consistently found in concentrations of 10(8) bacteria per milliliter of metal-working fluid during 1 year of observation. No increased frequency of respiratory infections or discomfort was related to the exposure to the infected fluid. The antibody response to the bacterium consisted predominantly of IgG1 and IgG2 antibodies. IgG2 antibodies dominated the antibody response to the lipopolysaccharide of the bacterium. IgA1 and IgA2 antibodies were also found. Smokers had significantly reduced antibody levels of all subclasses compared with nonsmokers. The antibody levels in smokers did not differ from levels of the unexposed control group. Analyses of the total serum immunoglobulin concentrations with respect to subclasses revealed that the total IgG2 levels were also significantly reduced in smokers. In nonsmokers, the age of the individuals influenced the antibody levels of the IgG1, IgG2, IgA1, and IgA2 subclasses, the levels decreasing with increasing age. For smokers, the correlation between age and antibody levels was only obvious for IgG2 antibodies. Decreased IgG2 antibody levels in the smokers were also accompanied by decreased FEV1 values (p less than 0.01). Subclass analysis of the antibody response to P. pseudoalcaligenes demonstrated that the subclass pattern for the whole bacterium differed from the pattern of the major cell wall component, the lipopolysaccharide. The significance of qualitative and quantitative differences in the subclass antibody response is discussed.
对暴露于含有嗜麦芽窄食单胞菌的雾化金属加工液的人群，研究了血清中IgG和IgA抗体反应的亚类分布。在1年的观察期内，该菌种在每毫升金属加工液中的浓度始终为10⁸个细菌。呼吸道感染频率或不适增加与接触受感染的液体无关。对该细菌的抗体反应主要由IgG1和IgG2抗体组成。IgG2抗体在对该细菌脂多糖的抗体反应中占主导。也发现了IgA1和IgA2抗体。与不吸烟者相比，吸烟者所有亚类的抗体水平显著降低。吸烟者的抗体水平与未接触的对照组水平无差异。对血清总免疫球蛋白浓度亚类的分析显示，吸烟者的总IgG2水平也显著降低。在不吸烟者中，个体年龄影响IgG1、IgG2、IgA1和IgA2亚类的抗体水平，水平随年龄增加而降低。对于吸烟者，年龄与抗体水平之间的相关性仅在IgG2抗体中明显。吸烟者IgG2抗体水平降低还伴有FEV1值降低（p<0.01）。对嗜麦芽窄食单胞菌抗体反应的亚类分析表明，针对整个细菌的亚类模式不同于主要细胞壁成分脂多糖的模式。讨论了亚类抗体反应中定性和定量差异的意义。
