Mattsby-Baltzer I, Edebo L, Järvholm B, Lavenius B
Department of Clinical Bacteriology, University of Göteborg, Sweden.
Int Arch Allergy Appl Immunol. 1989;88(3):304-11. doi: 10.1159/000234816.
Metal workers exposed to aerosol from metal-working fluid were examined with respect to serum antibody to the lipopolysaccharide (LPS) of Pseudomonas pseudoalcaligenes. During 1 year of observation this species grew at high concentrations in the fluid (approximately 10(8) CFU/ml), and the air surrounding the metal-working machines sometimes contained more than 10(5) CFU/m3. The levels of antibody belonging to the IgG and IgA classes were significantly higher than in blood donors and in workers newly employed. Comparison with employees not working in the machine hall showed a significantly higher IgG, but not IgA, antibody level. For IgM antibody no such difference was found. Among the exposed workers, non-smokers had significantly higher IgG antibody levels than smokers, whereas no such difference was established for IgA and IgM antibodies. In smokers the IgG antibody level seemed to decline in the long-range time, since there was a negative correlation between the time of employment with exposure to metal-working fluids and IgG antibody level. The antibody data indirectly demonstrate that P. pseudoalcaligenes grown in metal-working fluid penetrated the body surface of metal workers to yield an immune response most probably after inhalation of aerosol containing bacteria. In the metal workers this long-term exposure did not lead to any acute or chronic respiratory discomfort.
对接触金属加工液气溶胶的金属工人进行了关于抗嗜麦芽窄食单胞菌脂多糖（LPS）血清抗体的检测。在1年的观察期内，该菌在加工液中大量生长（浓度约为10⁸CFU/ml），金属加工机器周围空气中有时含有超过10⁵CFU/m³的该菌。IgG和IgA类抗体水平显著高于献血者和新入职工人。与不在机房工作的员工相比，IgG抗体水平显著更高，但IgA抗体水平无差异。未发现IgM抗体有此类差异。在接触的工人中，不吸烟者的IgG抗体水平显著高于吸烟者，而IgA和IgM抗体未发现此类差异。在吸烟者中，IgG抗体水平似乎随时间推移而下降，因为接触金属加工液的工作时间与IgG抗体水平呈负相关。抗体数据间接表明，在金属加工液中生长的嗜麦芽窄食单胞菌穿透了金属工人的体表，很可能是在吸入含菌气溶胶后引发了免疫反应。在这些金属工人中，这种长期接触并未导致任何急性或慢性呼吸道不适。
